Anti-Fibronectin antibody [A17] (ab26245)

Overview

• Product nameAnti-Fibronectin antibody [A17]See all Fibronectin primary antibodies ...
• Description
  Mouse monoclonal [A17] to Fibronectin
• SpecificityThere is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin).
• Tested applicationsWB, ELISA, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Chicken, Cow, Human
  
• Immunogen

  Tissue / cell preparation (Cow) - Lysed bovine corneal endothelial cells and extracellular matrix.

• EpitopeEpitope is located in the 120kD cell binding fragment.
• General notes

  
  

  This antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with thrombospondin directly coated onto the microtiter well.

Properties

• FormLiquid
• Storage instructionsStore at +4°C in the dark.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
• Concentration information loading...
• Purification notesProtein A/G purified
• Primary antibody notes This antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with thrombospondin directly coated onto the microtiter well.
• Clonality Monoclonal
• Clone numberA17
• MyelomaSp2/0
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Extracellular

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Fibronectin

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Extracellular Matrix

Applications

Target

• FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
  Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
• Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
• Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesContains 12 fibronectin type-I domains.
  Contains 2 fibronectin type-II domains.
  Contains 16 fibronectin type-III domains.
• Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
• Post-translational
  modificationsSulfated.
  It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
  Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
  Phosphorylation sites are present in the extracellular medium.
  Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02751 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396133 Chicken
  • Entrez Gene: 280794 Cow
  • Entrez Gene: 2335 Human
  • Omim: 135600 Human
  • SwissProt: P11722 Chicken
  • SwissProt: P07589 Cow
  • SwissProt: P02751 Human
  • Unigene: 203717 Human

• Alternative names
  CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody

  DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN 1 antibodyFN antibodyFN1 antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody

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Anti-Fibronectin antibody [A17] images

• 

  Immunocytochemistry/ Immunofluorescence - Fibronectin antibody [A17] (ab26245)This image is courtesy of an Abreview submitted by Dr Ann Van Soom
  ab26245 staining fibronectin in bovine cumulus-oocyte complexes  by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 0.1% serum for 30 minutes at 37°C. The primary antibody (1/100 in 10% goat serum in polylvinylpyrrolidone) was incubated with the sample for 1 hour at 37°C. An FITC-conjugated goat anti-mouse polyclonal (1/100) was used as the secondary.  Chromatin was stained red by using the nuclear DNA stain propidium iodide.

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