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Tissue/ cell preparation (Cow) - Lysed bovine corneal endothelial cells and extracellular matrix.
This antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with thrombospondin directly coated onto the microtiter well.
Our Abpromise guarantee covers the use of ab26245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/100. Predicted molecular weight: 263 kDa. See Underwood et al J Cell Sci reference.|
|ELISA||1/30000. See Underwood et al J Cell Sci reference and J Immunol Methods reference.|
|IHC-Fr||Use at an assay dependent concentration.
|ICC/IF||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab26245 staining Fibronectin in Human MCF-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% BSA for 1 hours at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 5% BSA) for 18 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody. Blue - DAPI, red - rhodamine phalloidin.
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