Overview

  • Product nameAnti-Fibronectin antibody [A17]See all Fibronectin primary antibodies ...
  • Description
    Mouse monoclonal [A17] to Fibronectin
  • SpecificityThere is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin).
  • Tested applicationsIP, IHC-P, WB, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Chicken, Cow, Human
  • Immunogen

    Tissue/ cell preparation (Cow) - Lysed bovine corneal endothelial cells and extracellular matrix.

  • EpitopeEpitope is located in the 120kD cell binding fragment.
  • General notes


    This antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with thrombospondin directly coated onto the microtiter well.

Properties

Applications

Our Abpromise guarantee covers the use of ab26245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 263 kDa. See Underwood et al J Cell Sci reference.
ELISA 1/30000. See Underwood et al J Cell Sci reference and J Immunol Methods reference.
IHC-Fr Use at an assay dependent concentration.

PLP-fixed.

ICC/IF Use at an assay dependent concentration.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN 1 antibody
    • FN 1 antibody
    • FN antibody
    • FN1 antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Transformation sensitive protein. antibody
    • Transformation sensitive protein. antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody [A17] images

  • ab26245 staining Fibronectin in Human MCF-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% BSA for 1 hours at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 5% BSA) for 18 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody. Blue - DAPI, red - rhodamine phalloidin.

    See Abreview

  • ab26245 staining fibronectin in bovine cumulus-oocyte complexes  by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 0.1% serum for 30 minutes at 37°C. The primary antibody (1/100 in 10% goat serum in polylvinylpyrrolidone) was incubated with the sample for 1 hour at 37°C. An FITC-conjugated goat anti-mouse polyclonal (1/100) was used as the secondary.  Chromatin was stained red by using the nuclear DNA stain propidium iodide.

    See Abreview

References for Anti-Fibronectin antibody [A17] (ab26245)

This product has been referenced in:
  • Küttner V  et al. Global remodelling of cellular microenvironment due to loss of collagen VII. Mol Syst Biol 9:657 (2013). Read more (PubMed: 23591773) »
  • Yang G  et al. Enhancement of tenogenic differentiation of human adipose stem cells by tendon-derived extracellular matrix. Biomaterials 34:9295-306 (2013). WB ; Cow . Read more (PubMed: 24044998) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (MCF-7 Cells)
Specification MCF-7 Cells
Permeabilization Yes - 0.1%Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 02 2013

Thank you for contacting us.

Normally antibodies are OK for 1 year at 4C however it varies antibodies to antibodies so we always recommend following the guidelines provided on datasheet.

The ab78030 and ab26245 is recommended to be st...

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Thank you very much for your interest our AbTrial program.


To our knowledge, ab26245 Anti-Fibronectin antibody [A17] has not been tested in bovine samples. However by participating in our AbTrial program you can now use our products in ...

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Thank you for contacting us. The ab6328 data we have for chicken reactivity is a chicken embryo IHC review submitted by a customer on April 3, 2008. The review is linked on the datasheet: http://www.abcam.com/index.html?pageconfig=revie...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (cumulus-oocyte complexes, cumulus cells, oocytes)
Specification cumulus-oocyte complexes, cumulus cells, oocytes
Fixative Paraformaldehyde
Permeabilization Yes - triton-X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 37°C
Username

Dr. Ann Van Soom

Verified customer

Submitted Nov 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon cancer cell line Caco2)
Loading amount 30 µg
Specification Colon cancer cell line Caco2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Username

Abcam user community

Verified customer

Submitted Nov 07 2006

Thank you for your enquiry. I have received feedback from the source of ab26245 which wished to clarify the following information: Cellular fibronectin is indeed mentioned in the relevance paragraph of the antibody however this was to provide full i...

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The soluble plasma isoform of fibronectin consists of two very similar subunits and the insoluble cellular for is a multimer of the same subunits, we expect the epitope of ab26245(i.e. somewhere on the 120kD cell binding fragment, away from the RGDS se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"