Anti-Fibronectin antibody (Biotin) (ab6584)
Overview
- Product nameAnti-Fibronectin antibody (Biotin)See all Fibronectin primary antibodies ...
- DescriptionRabbit polyclonal to Fibronectin (Biotin)
- ConjugationBiotin
- Conjugation notesBiotin
- Specificitynegligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
- Tested applicationsELISA, IHC-P, IP, WB, ICC more details
- Species reactivityReacts with: Mouse, Cow, Human
Predicted to work with: all Mammals - Immunogen
Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
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Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
- Primary antibody notes This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab6584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/4000 - 1/8000. |
| IHC-P | IHC-P: 1/50 - 1/200. |
| IP | IP: Use at an assay dependent dilution. |
| WB | WB: 1/5000 - 1/10000. |
| ICC | ICC: Use at an assay dependent dilution. |
Target
- FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. - Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
- Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesContains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. - Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
- Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylation sites are present in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. - Cellular localizationSecreted > extracellular space > extracellular matrix.
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Database links
- Entrez Gene: 280794 Cow
- Entrez Gene: 2335 Human
- Entrez Gene: 14268 Mouse
- Omim: 135600 Human
- SwissProt: P07589 Cow
- SwissProt: P02751 Human
- SwissProt: P11276 Mouse
- Unigene: 203717 Human
Target information above from: UniProt accession
P02751
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody
- DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN antibodyFN1 antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody
see all
Anti-Fibronectin antibody (Biotin) images
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Immunocytochemistry/ Immunofluorescence - Fibronectin antibody (Biotin) (ab6584)Human Aortic Endothelial Cells grown in MCDB 131+ SmGM-2 media were seeded on a PET membrane. After one week, the cells were blocked with PBS containing 0.1% BSA for 15 min. The membrane was incubated with primary antibody (ab6584) for 30 min and then with secondary antibody Alexa 488 for 1 hr.
This picture was kindly supplied as part of the review submitted by Gary Nackman and Ildiko Entersz.
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Western blot - Anti-Fibronectin antibody (Biotin) (ab6584)Anti-Fibronectin antibody (Biotin) (ab6584) at 1 µg/ml +Fibronectin protein (Human) (ab81743) at 0.001 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody (Biotin) (ab6584)Image from Sabari J et al., PLoS One. 2011;6(9):e24810. Epub 2011 Sep 30. Fig 5.; doi:10.1371/journal.pone.0024810; September 30, 2011, PLoS ONE 6(9): e24810. doi:10.1371/journal.pone.0024810Immunofluorescence analysis of U87-MG cells staining Fibronectin (red) with ab6584.
Cells were treated with 10-6M Dexamethasone, before blocking and incubating with primary antibody for 1 hour at room temperature. An AlexaFluor®568-conjugated anti-rabbit IgG was used as the secondary antibody.
References for Anti-Fibronectin antibody (Biotin) (ab6584)
This product has been referenced in:
- Martin TA et al. The generation of biomolecular patterns in highly porous collagen-GAG scaffolds using direct photolithography. Biomaterials 32:3949-57 (2011). ICC/IF ; Mouse . Read more (PubMed: 21397322) »
- Sabari J et al. Fibronectin matrix assembly suppresses dispersal of glioblastoma cells. PLoS One 6:e24810 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21980357) »
