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Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Our Abpromise guarantee covers the use of ab6584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/4000 - 1/8000.|
|IHC-P||1/50 - 1/200.|
|IP||Use at an assay dependent dilution.|
|WB||1/5000 - 1/10000.|
|ICC||Use at an assay dependent dilution.|
Human Aortic Endothelial Cells grown in MCDB 131+ SmGM-2 media were seeded on a PET membrane. After one week, the cells were blocked with PBS containing 0.1% BSA for 15 min. The membrane was incubated with primary antibody (ab6584) for 30 min and then with secondary antibody Alexa 488 for 1 hr.
This picture was kindly supplied as part of the review submitted by Gary Nackman and Ildiko Entersz.
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