Overview

  • Product nameAnti-Fibronectin antibody (Biotin)See all Fibronectin primary antibodies ...
  • Description
    Rabbit polyclonal to Fibronectin (Biotin)
  • ConjugationBiotin
  • Conjugation notesBiotin
  • Specificitynegligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
  • Tested applicationsELISA, IHC-P, IP, WB, ICC more details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: all Mammals
  • Immunogen

    Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE

  • General notes


    This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
  • Primary antibody notes This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/4000 - 1/8000.
IHC-P 1/50 - 1/200.
IP Use at an assay dependent dilution.
WB 1/5000 - 1/10000.
ICC Use at an assay dependent dilution.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN 1 antibody
    • FN antibody
    • FN1 antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Transformation sensitive protein. antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody (Biotin) images

  • Human Aortic Endothelial Cells grown in MCDB 131+ SmGM-2 media were seeded on a PET membrane. After one week, the cells were blocked with PBS containing 0.1% BSA for 15 min. The membrane was incubated with primary antibody (ab6584) for 30 min  and then with secondary antibody Alexa 488 for 1 hr.

    This picture was kindly supplied as part of the review submitted by Gary Nackman and Ildiko Entersz.

  • Anti-Fibronectin antibody (Biotin) (ab6584) at 1 µg/ml + Human Fibronectin full length protein (ab81743) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
  • Immunofluorescence analysis of U87-MG cells staining Fibronectin (red) with ab6584.

    Cells were treated with 10-6M Dexamethasone, before blocking and incubating with primary antibody for 1 hour at room temperature. An AlexaFluor®568-conjugated anti-rabbit IgG was used as the secondary antibody.

References for Anti-Fibronectin antibody (Biotin) (ab6584)

This product has been referenced in:
  • Martin TA  et al. The generation of biomolecular patterns in highly porous collagen-GAG scaffolds using direct photolithography. Biomaterials 32:3949-57 (2011). ICC/IF ; Mouse . Read more (PubMed: 21397322) »
  • Sabari J  et al. Fibronectin matrix assembly suppresses dispersal of glioblastoma cells. PLoS One 6:e24810 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21980357) »

See all 9 Publications for this product

Product Wall

Thank you for your enquiry about our fibronectin antibodies. The antibody ab6584 is biotin conjugated and has been successfully used in IF (ICC) by a user of the antibody who left a review which can be accessed via the online datasheet. The reviewer di...

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For testing 1ug of antigen, the minimum of antibody used is 1:8000. The maximum is 1:4000. We don't have other data to show the sensitivity. If you have any additional questions, please contact us again.

1:4000 to 1:8000 dilution can detect 1.0ug of Fibronectin. If you have any additional questions, please contact us again.

I have not found reports of staining of fibronectin in the cytoplasm of plasma cells, however it is known that plasma cells adhere to fibronectin (the fibronectin inducing not only the adherence and spread of plasma cells but also their migration). ...

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Thank you for your enquiry. We would like to confirm that the concentration is 2.5mg/mL, so 40uL will give 100ug of antibody.

This antibody does not require antigen retrieval but it may increase staining. We normally recommend the pressure cooker method of antigen retrieval.

450 kDa non-reduced 225 kDa fully reduced

This antibody will cross-ract with mouse fibronectin using formalin fixed paraffin embedded tissues.

Yes, this antibody is available in an unconjugated form, ab299

The extracellular matrix proteins are well conserved, particularly between mammals, so human will cross-react with pig.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"