You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Fibronectin antibody [HFN 7.1] - BSA and Azide free (ab80923)

Submit an Abreview Q&A (2)

Overview

  • Product nameAnti-Fibronectin antibody [HFN 7.1] - BSA and Azide freeSee all Fibronectin primary antibodies ...
  • Description
    Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free
  • Specificityab80923 is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface
  • Tested applicationsSandwich ELISA, WB, IP, Blocking more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, Human Fibronectin, purified from serum by affinity chromatography on gelatinsepharose.

  • EpitopePeptide core
  • Positive controlSW156 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab80923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibronectin (ab299). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Fibronectin (ab299) as Detection.
WB WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 263 kDa.
IP IP: Use at 2 µg/mg of lysate. (Native and denatured - use Protein G)
Blocking BL: Use at an assay dependent dilution.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P02751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody
        DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyFibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyUgl-Y3 antibody
      see all

    Anti-Fibronectin antibody [HFN 7.1] - BSA and Azide free images

    Protocols

    References for Anti-Fibronectin antibody [HFN 7.1] - BSA and Azide free (ab80923)

    ab80923 has not yet been referenced specifically in any publications.

    Publishing research using ab80923 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab80923 Submit a Question for ab80923

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First

    Please recommend a pair of antibodies and the antigen that will give a strong signal in sandwich ELISA. The antigen can be anything, we just want a strong signal. We may want to biotinylate the capture antibody, and we may need a gold-conjugated secondary...

    Read More

    I found a good set of products that gives a strong sandwich ELISA signal.

    Capture: ab80923

    Detection: ab299

    Antigen: ab81743

    Click here (or use the following: http://www.abcam.com/index.html?datasheet=80923). Read More

    thank you very much for your email and for the suggestions. However, for our experiments we are looking for a protein that is released into the extracellular matrix of the body. If I am not mistaken OCT-4 seems to stay within the cell. Could you recommend...

    Read More


    I've included a list of capture, detection, protein, and fluorescent-conjugated secondary below against Fibronectin. The capture and detection antibodies have been tested and guaranteed to work in sandwich ELISA to detect human fibronectin:

    ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"