Overview
- Product nameAnti-Fibronectin antibody [IST-10]See all Fibronectin primary antibodies ...
- DescriptionMouse monoclonal [IST-10] to Fibronectin
- Tested applicationsIHC-Fr, WB more details
- Species reactivityReacts with: Human
- Immunogen
Purified fibronectin.
- Epitopeab78288 recognizes an epitope contained within the gelatin binding domain of fibronectin
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone numberIST-10
- IsotypeIgG1
- Light chain typekappa
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab78288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 263 kDa. |
Target
- FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. - Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
- Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesContains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. - Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
- Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylation sites are present in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. - Cellular localizationSecreted > extracellular space > extracellular matrix.
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Database links
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
- Unigene: 203717 Human
Target information above from: UniProt accession
P02751
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody
- DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyFibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyUgl-Y3 antibody
see all
References for Anti-Fibronectin antibody [IST-10] (ab78288)
ab78288 has not yet been referenced specifically in any publications.
