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Our Abpromise guarantee covers the use of ab6328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|RIA||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.
Concentration of IST-9 in IHC-fr - depending on the system - is 0.1 to 2 micrograms per ml.
IST-9 works very well using all iHC-fr procedures.
|Other||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration. PubMed: 20643910|
|IHC-P||1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Concentration of IST-9 in IHC-P is - depending on the system - 2-10 micrograms per ml.
ab6328 staining Fibronectin in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a Tris-EDTA buffer pH9.0. Samples were incubated with primary antibody (1/50 in blocking buffer) for 30 minutes at 20°C. An undiluted HRP-conjugated Goat anti-mouse polyclonal was used as the secondary antibody.
ab6328 staining fibronectin in human esophageal epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 1% Triton X-100 in PBS and blocked with 0.25% DAKO serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in DAKO antibody diluent) for 2 hours at 28°C. ab6785 (1/800) was used as the secondary antibody.
This photo shows Fibronectin localization in the extracellular matrix of monolayer culture of human esophageal epithelial cells (Het-1A). Nuclei were counterstained with propidium iodide.
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