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Anti-Fibronectin antibody [IST-9] (ab6328)

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Overview

Product name

Anti-Fibronectin antibody [IST-9]
See all Fibronectin products (25) ...

Description

Mouse monoclonal [IST-9] to Fibronectin

Specificity

This antibody reacts with an epitope (PEDGIHELFP)located in the ED-A sequence of cellular fibronectin (it therefore only detects the cFN and not extracellular FN (plasma FN)). The ED-A segment can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. In transformed or tumour derived cells the ED-A segment is about 10-times higher than in FN from normal fibroblasts, and it may therefore be a significant marker for malignancy (Borsi et al., and others).

Tested applications

WB, RIA, IHC-Fr, ICC/IF, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Chicken, Cow, Dog, Human, Pig, Monkey

Predicted to work with

Rabbit

Immunogen

A region in the ED-A domain of human cellular fibronectin.

Positive control

normal foetal lung fibroblasts (cell line MRC5), skin, rat or human kidney (see reviews)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Constituent: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

IST-9

Isotype

IgG1

Research areas

Developmental Biology >> Lineage specification >> Ectoderm
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Neural Stem Cells >> Extracellular
Stem Cells >> Lineage Markers >> Ectoderm
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Fibronectin
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Fibronectin antibody [IST-9] (ab6328)Immunocytochemistry/ Immunofluorescence - Fibronectin antibody [IST-9] (ab6328) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Tissue specificity

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Involvement in disease

Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life.

Sequence similarities

Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains.

Developmental stage

Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.

Post-translational
modifications

Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylation sites are present in the extracelllular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP02751 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CIG antibody
  • Cold insoluble globulin antibody
  • Cold-insoluble globulin antibody
  • DKFZp686F10164 antibody
  • DKFZp686H0342 antibody
  • DKFZp686I1370 antibody
  • DKFZp686O13149 antibody
  • ED B antibody
  • fibronectin 1 antibody
  • FINC antibody
  • FINC_HUMAN antibody
  • FN 1 antibody
  • FN antibody
  • FN1 antibody
  • FN1 antibody
  • FNZ antibody
  • GFND antibody
  • GFND2 antibody
  • LETS antibody
  • Migration stimulating factor antibody
  • MSF antibody
  • Transformation sensitive protein. antibody
  • Ugl-Y3 antibody
see all

Database links

see all

Anti-Fibronectin antibody [IST-9] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)

This picture shows formalin fixed paraffin embedded rat kidney stained with ab6328 at a dilution of 1:100 after proteinase K digestion.The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)

Ab6328 positively staining paraffin fixed canine myocardial infarct. tissue (1/200). The images demonstrate the time course of fibronectin deposition in reperfused canine myocaridal infarcts.
a: 24 hrs
b: 7 days
c: 28 days of reperfusion.

Secondary: Biotin conjugated goat anti mouse (5 µg/ml). Detection was achieved using DAB.

This image is an edited version of an image submitted courtesy of an Abreview by Marcin Dobaczewski. We do not have any further information relating to this image.

See Abreview

  Immunocytochemistry/ Immunofluorescence - Fibronectin antibody [IST-9] (ab6328)

Immunocytochemistry/ Immunofluorescence - Fibronectin antibody [IST-9] (ab6328)

ab6328 staining human arterial endothelial cells by ICC/IF.  Cells were PFA fixed, permeabilized with 0.05% Triton X-100 and blocked with 0.2% BSA for 1 hour at 25°C  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C.  An Alexa Fluor® 488 conjugated donkey anti-mouse antibody, diluted 1/400, was used as the secondary.

This image is courtesy of an Abreview submitted by Mrs Elena Astanina

See Abreview

  Western blot - Anti-Fibronectin antibody [IST-9] (ab6328)

Western blot - Anti-Fibronectin antibody [IST-9] (ab6328)

Anti-Fibronectin antibody [IST-9] (ab6328) at 1/1000 dilution + whole cell lysate prepared from human platelets ( treated with ADP for 30 minutes) at 20 µg

Secondary
HRP conjugated donkey anti-mouse polyclonal at 1/10000 dilution
developed using the ECL technique

Exposure time : 2 minutes

Blocked with 5% milk for 1 hour at 22°C.

Image courtesy of Dr Mahesh Shivananjappa by Abreview.

See Abreview

Protocols

  • Recommended RIA protocol with ab6328

    • References for Anti-Fibronectin antibody [IST-9] (ab6328)

    This product has been referenced in:

    • Indra I  et al. An in vitro correlation of mechanical forces and metastatic capacity. Phys Biol 8:015015 (2011). WB; Mouse.Read more (PubMed: 21301068) »
    • Chou CY  et al. A Crucial Sequence for Transglutaminase Type 2 Extracellular Trafficking in Renal Tubular Epithelial Cells Lies in Its N-terminal {beta}-Sandwich Domain. J Biol Chem 286:27825-35 (2011). WB.Read more (PubMed: 21652693) »

    See all 32 publications for this product

    Publishing research using ab6328? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"