Anti-Fibronectin antibody [IST-9] (ab6328)

Overview

• Product nameAnti-Fibronectin antibody [IST-9]See all Fibronectin primary antibodies ...
• Description
  Mouse monoclonal [IST-9] to Fibronectin
• SpecificityThis antibody reacts with an epitope (PEDGIHELFP)located in the ED-A sequence of cellular fibronectin (it therefore only detects the cFN and not extracellular FN (plasma FN)). The ED-A segment can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. In transformed or tumour derived cells the ED-A segment is about 10-times higher than in FN from normal fibroblasts, and it may therefore be a significant marker for malignancy (Borsi et al., and others).
• Tested applicationsWB, RIA, ELISA, IHC-Fr, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Pig, Monkey
  Predicted to work with: Rabbit
• Immunogen

  A region in the ED-A domain of human cellular fibronectin.

• Positive controlnormal foetal lung fibroblasts (cell line MRC5), skin, rat or human kidney (see reviews)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferConstituent: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone numberIST-9
• IsotypeIgG1
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Extracellular

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Fibronectin

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Extracellular Matrix

Applications

Target

• FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
  Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
• Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
• Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesContains 12 fibronectin type-I domains.
  Contains 2 fibronectin type-II domains.
  Contains 16 fibronectin type-III domains.
• Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
• Post-translational
  modificationsSulfated.
  It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
  Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
  Phosphorylation sites are present in the extracellular medium.
  Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02751 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396133 Chicken
  • Entrez Gene: 280794 Cow
  • Entrez Gene: 2335 Human
  • Entrez Gene: 14268 Mouse
  • Entrez Gene: 25661 Rat
  • Omim: 135600 Human
  • SwissProt: P11722 Chicken
  • SwissProt: P07589 Cow

  • SwissProt: P02751 Human
  • SwissProt: P11276 Mouse
  • SwissProt: P04937 Rat
  • Unigene: 203717 Human

  see all

• Alternative names
  CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody

  DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN antibodyFN1 antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody

  see all

Anti-Fibronectin antibody [IST-9] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] (ab6328)This image is courtesy of an Abreview submitted by Rudolf Jung

  ab6328 staining Fibronectin in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a Tris-EDTA buffer pH9.0. Samples were incubated with primary antibody (1/50 in blocking buffer) for 30 minutes at 20°C. An undiluted HRP-conjugated Goat anti-mouse  polyclonal was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)
  This picture shows formalin fixed paraffin embedded rat kidney stained with ab6328 at a dilution of 1:100 after proteinase K digestion.The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibronectin antibody [IST-9] (ab6328)
  Ab6328 positively staining paraffin fixed canine myocardial infarct. tissue (1/200). The images demonstrate the time course of fibronectin deposition in reperfused canine myocaridal infarcts.
  a: 24 hrs
  b: 7 days
  c: 28 days of reperfusion.
  
  Secondary: Biotin conjugated goat anti mouse (5 µg/ml). Detection was achieved using DAB.
  
  This image is an edited version of an image submitted courtesy of an Abreview by Marcin Dobaczewski. We do not have any further information relating to this image.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Fibronectin antibody [IST-9] (ab6328)This image is courtesy of an Abreview submitted by Mrs Elena Astanina

  ab6328 staining human arterial endothelial cells by ICC/IF.  Cells were PFA fixed, permeabilized with 0.05% Triton X-100 and blocked with 0.2% BSA for 1 hour at 25°C  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C.  An Alexa Fluor® 488 conjugated donkey anti-mouse antibody, diluted 1/400, was used as the secondary.

  See Abreview

• 

  Western blot - Anti-Fibronectin antibody [IST-9] (ab6328)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
  Anti-Fibronectin antibody [IST-9] (ab6328) at 1/1000 dilution + whole cell lysate prepared from human platelets ( treated with ADP for 30 minutes) at 20 µg
  
  Secondary
  HRP conjugated donkey anti-mouse polyclonal at 1/10000 dilution
  developed using the ECL technique
  
  Exposure time : 2 minutes

  Image courtesy of Dr Mahesh Shivananjappa by Abreview.

  Blocked with 5% milk for 1 hour at 22°C.

  See Abreview

• 

  Western blot - Anti-Fibronectin antibody [IST-9] (ab6328)This image is courtesy of an anonymous Abreview
  All lanes : Anti-Fibronectin antibody [IST-9] (ab6328) at 1/2000 dilution
  
  Lane 1 : Human dermal fibroblast whole cell lysate, untreated
  Lane 2 : Human dermal fibroblast whole cell lysate, treated with TGF beta 1
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-conjugated Goat anti-mouse monoclonal at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 15 seconds

  This image is courtesy of an anonymous Abreview

  Blocked with 5% Milk for 1 hour at 20°C.

  See Abreview

Protocols

• Recommended RIA protocol with ab6328