Overview

  • Product nameAnti-Fibronectin antibodySee all Fibronectin primary antibodies ...
  • Description
    Sheep polyclonal to Fibronectin
  • Specificityab23516 recognises fibronectin, 460-500kD glycoprotein involved in tissue organisation and wound repair.
  • Tested applicationsIHC-Fr, Immunodiffusion, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Horse, Guinea pig, Cat, Dog, Human, Pig

    Does not react with

    Mouse, Rabbit, Goat, Chicken, Cow
  • Immunogen

    Full length native human fibronectin protein (purified).

  • Positive control
    • Kidney

Properties

Applications

Our Abpromise guarantee covers the use of ab23516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Immunodiffusion
ELISA
  • Application notesID: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
      Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
    • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
    • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
    • Sequence similaritiesContains 12 fibronectin type-I domains.
      Contains 2 fibronectin type-II domains.
      Contains 16 fibronectin type-III domains.
    • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
    • Post-translational
      modifications
      Sulfated.
      It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
      Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
      Phosphorylation sites are present in the extracellular medium.
      Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CIG antibody
      • Cold insoluble globulin antibody
      • Cold-insoluble globulin antibody
      • DKFZp686F10164 antibody
      • DKFZp686H0342 antibody
      • DKFZp686I1370 antibody
      • DKFZp686O13149 antibody
      • ED B antibody
      • fibronectin 1 antibody
      • FINC antibody
      • FINC_HUMAN antibody
      • FN 1 antibody
      • FN 1 antibody
      • FN antibody
      • FN1 antibody
      • FN1 antibody
      • FNZ antibody
      • GFND antibody
      • GFND2 antibody
      • LETS antibody
      • LETS antibody
      • Migration stimulating factor antibody
      • MSF antibody
      • Transformation sensitive protein. antibody
      • Transformation sensitive protein. antibody
      • Ugl-Y3 antibody
      see all

    Anti-Fibronectin antibody images

    • ab23516 staining Fibronectin in porcine trachea by Immunohistochemistry (Frozen sections).
      Tissue was fixed in acetone and blocked using 1% BSA with 10% donkey serum for 2 hours at room temperature. Samples were then incubated with ab23516 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor® 555 donkey anti-sheep IgG (H+L), used at a 1/200 dilution.

      See Abreview

    References for Anti-Fibronectin antibody (ab23516)

    ab23516 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Pig Tissue sections (Trachea)
    Specification Trachea
    Fixative Acetone
    Permeabilization No
    Blocking step 1% BSA with 10% Donkey Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

    Ms. Leanne Partington

    Verified customer

    Submitted Jul 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"