Anti-Fibronectin antibody (ab23749)

Overview

  • Product nameAnti-Fibronectin antibody
    See all Fibronectin primary antibodies
  • Description
    Rabbit polyclonal to Fibronectin
  • SpecificityChicken fibronectin 100%, chicken collagen type I, III and V <0.1%
  • Tested applicationsICC/IF, IHC-P, ELISA, RIAmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Purified fibronectin from chicken plasma.

  • Positive control
    • Chicken skin
  • General notes


    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Properties

Applications

Our Abpromise guarantee covers the use of ab23749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
ELISA
RIA
  • Application notesELISA: Use at an assay dependent dilution.
    IF: 1/40.
    IHC-P: 1/200 - 1/600. Incubation time:60 min at RT or 2-8°C over night
    RIA: 1/10000.

    After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
      Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
    • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
    • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
    • Sequence similaritiesContains 12 fibronectin type-I domains.
      Contains 2 fibronectin type-II domains.
      Contains 16 fibronectin type-III domains.
    • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
    • Post-translational
      modifications
      Sulfated.
      It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
      Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
      Phosphorylation sites are present in the extracellular medium.
      Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CIG antibody
      • Cold insoluble globulin antibody
      • Cold-insoluble globulin antibody
      • DKFZp686F10164 antibody
      • DKFZp686H0342 antibody
      • DKFZp686I1370 antibody
      • DKFZp686O13149 antibody
      • ED B antibody
      • fibronectin 1 antibody
      • FINC antibody
      • FINC_HUMAN antibody
      • FN 1 antibody
      • FN 1 antibody
      • FN antibody
      • FN1 antibody
      • FN1 antibody
      • FNZ antibody
      • GFND antibody
      • GFND2 antibody
      • LETS antibody
      • LETS antibody
      • Migration stimulating factor antibody
      • MSF antibody
      • Transformation sensitive protein. antibody
      • Transformation sensitive protein. antibody
      • Ugl-Y3 antibody
      see all

    References for Anti-Fibronectin antibody (ab23749)

    ab23749 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. The ab6328 data we have for chicken reactivity is a chicken embryo IHC review submitted by a customer on April 3, 2008. The review is linked on the datasheet: http://www.abcam.com/index.html?pageconfig=revie...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"