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Anti-Fibronectin antibody (ab23749)

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Overview

  • Product nameAnti-Fibronectin antibodySee all Fibronectin primary antibodies ...
  • Description
    Rabbit polyclonal to Fibronectin
  • SpecificityChicken fibronectin 100%, chicken collagen type I, III and V <0.1%
  • Tested applicationsICC/IF, IHC-P, ELISA, RIA more details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Purified fibronectin from chicken plasma.

  • Positive controlChicken skin
  • General notes


    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Properties

Applications

Our Abpromise guarantee covers the use of ab23749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
IHC-P
ELISA
RIA
  • Application notesELISA: Use at an assay dependent dilution.
    IF: 1/40.
    IHC-P: 1/200 - 1/600. Incubation time:60 min at RT or 2-8°C over night
    RIA: 1/10000.

    After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
      Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
    • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
    • Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • Sequence similaritiesContains 12 fibronectin type-I domains.
      Contains 2 fibronectin type-II domains.
      Contains 16 fibronectin type-III domains.
    • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
    • Post-translational
      modifications      Sulfated.
      It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
      Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
      Phosphorylation sites are present in the extracellular medium.
      Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.

      • Target information above from: UniProt accession P02751 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody
          DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN 1 antibodyFN antibodyFN1 antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody
        see all

      References for Anti-Fibronectin antibody (ab23749)

      ab23749 has not yet been referenced specifically in any publications.

      Publishing research using ab23749 ? Please let us know so that we can cite the reference in this datasheet

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      I am looking for a good primary antibody against fibronectin in the chick embryo. The antibody ab6328 has a listed reactivity with chicken. However, I couldn't find any mention of it in the references or in the datasheet. For what reason do you expect...

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      Thank you for contacting us. The ab6328 data we have for chicken reactivity is a chicken embryo IHC review submitted by a customer on April 3, 2008. The review is linked on the datasheet: http://www.abcam.com/index.html?pageconfig=reviews&...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"