Overview

  • Product nameAnti-Fibronectin antibodySee all Fibronectin primary antibodies ...
  • Description
    Rabbit polyclonal to Fibronectin
  • SpecificityMurine fibronectin 100%, murine collagen type I, III and IV <0.1%, murine laminin <0.1%
  • Tested applicationsELISA, RIA, ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Purified fibronectin from murine plasma.

  • Positive control
    • Mouse skin
  • General notes


    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Properties

Applications

Our Abpromise guarantee covers the use of ab23750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
RIA
ICC/IF
IHC-Fr
WB
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent concentration.
    IF: 1/40.
    IHC-P: 1/200 - 1/600.
    IHC-Fr: Use at an assay dependent dilution.
    RIA: 1/10000.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 265 kDa (predicted molecular weight: 263 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
      Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
    • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
    • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
    • Sequence similaritiesContains 12 fibronectin type-I domains.
      Contains 2 fibronectin type-II domains.
      Contains 16 fibronectin type-III domains.
    • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
    • Post-translational
      modifications
      Sulfated.
      It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
      Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
      Phosphorylation sites are present in the extracellular medium.
      Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CIG antibody
      • Cold insoluble globulin antibody
      • Cold-insoluble globulin antibody
      • DKFZp686F10164 antibody
      • DKFZp686H0342 antibody
      • DKFZp686I1370 antibody
      • DKFZp686O13149 antibody
      • ED B antibody
      • fibronectin 1 antibody
      • FINC antibody
      • FINC_HUMAN antibody
      • FN 1 antibody
      • FN 1 antibody
      • FN antibody
      • FN1 antibody
      • FN1 antibody
      • FNZ antibody
      • GFND antibody
      • GFND2 antibody
      • LETS antibody
      • LETS antibody
      • Migration stimulating factor antibody
      • MSF antibody
      • Transformation sensitive protein. antibody
      • Transformation sensitive protein. antibody
      • Ugl-Y3 antibody
      see all

    Anti-Fibronectin antibody images

    • Anti-Fibronectin antibody (ab23750) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 263 kDa
      Observed band size : 265 kDa (why is the actual band size different from the predicted?)
    • ab23750 staining Fibronectin in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence. Cells were formaldehyde fixed, blocked in 2% BSA for 45 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, was used undiluted as secondary. The nuclei were stained with DAPI.

    • ab23750 staining fibronectin in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary antibody.

      See Abreview

    • ab23750 (2ug/ml) staining Fibronectin in human aorta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining Fibronectin in the cytoplasm and extracellularly in the connective tissue.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • ICC/IF image of ab23750 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23750, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab23750 staining Fibronectin in Rat skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Immunoblock® (5 µg/ml) for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in diluent) for 1 hour 30 minutes at 25°C. An undiluted Goat anti-rabbit HRP/DAB polydetector polyclonal was used as the secondary antibody.

      See Abreview

    References for Anti-Fibronectin antibody (ab23750)

    This product has been referenced in:
    • Stratmann AT  et al. Establishment of a human 3D lung cancer model based on a biological tissue matrix combined with a Boolean in silico model. Mol Oncol 8:351-65 (2014). Human . Read more (PubMed: 24388494) »
    • Meng F  et al. Induction of retinal ganglion-like cells from fibroblasts by adenoviral gene delivery. Neuroscience 250:381-93 (2013). Read more (PubMed: 23856066) »

    See all 18 Publications for this product

    Product Wall

    ELISA

    Good
    Abreviews
    Application ELISA
    Sample Human Serum (blood)
    Specification blood
    Type Sandwich (Detection)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Yeri-Alice Rim

    Verified customer

    Submitted May 23 2013

    Thank you for contacting us.

    This antibody has been characterised in Western Blot using 5% BSA as a blocking agent (http://www.abcam.com/Fibronectin-antibody-ab23750.html#Fibronectin-Primary-antibodies-ab23750-2.jpg). It has also been reported...

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    r/>
    I can confirm the Safety Data Sheets can be found on the online datasheets. Near the top right hand side of the datasheet, there are two 'buttons', one for Datasheet PDF and the one underneath says SDS. Click on the SDS button to link to the d...

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    Thank you for contacting us.

    I do understand and appreciate the amount of time you have spent it the laboratory. I am sorry to hear you are disappointed with our service.


    If a particular antibody causes problems we do encourag...

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    Thank you for taking time to answer my questions.


    Having reviewed the additional protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologi...

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    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (skin)
    Specification skin
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization No
    Blocking step immunoblock as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 13 2012

    Thank you for your enquiry.

    Although we produce many antibodies in house, I am sorry to confirm that this particular antibody has been tested externally, and regrettably we have no images of western blot on mouse samples.

    However,...

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    Indeed, I can confirm that the immunogen shares a sequence similarity of 97% with rat and it is therefore likely that the antibody cross-reacts. DISCOUNT CODE: xxx Expiration date: dd mm 2012 I am very pleased to hear you would like to accept our offer...

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    I recently "published" your Abreview on ab23750 on our website. Thank you for sharing your data with us! I just wanted to follow up with as you had rated your experience with this antibody as 'below average'. Since the staining was specific but lacked ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"