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Purified fibronectin from murine plasma.
This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.
Our Abpromise guarantee covers the use of ab23750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 265 kDa (predicted molecular weight: 263 kDa).|
|IHC-P||1/200 - 1/600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab23750 staining Fibronectin in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence. Cells were formaldehyde fixed, blocked in 2% BSA for 45 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, was used undiluted as secondary. The nuclei were stained with DAPI.
Immunocytochemical analysis of PFA fixed murine fibroblast tissue sections, labelling Fibronectin with ab23750 at 1/200 incubated for 13 hours at 4°C in TBS with 0.1% BSA. Blocking was with 1% BSA for 1 hour at room temperature. The secondary was a chicken anti-rabbit polyclonal Alexa Fluor® 488 at 1/400.