Overview

  • Product nameAnti-Fibronectin antibodySee all Fibronectin primary antibodies ...
  • Description
    Rabbit polyclonal to Fibronectin
  • SpecificityFibronectin 100%, collagen type I, III and IV <0.1%, elastin <0.1%
  • Tested applicationsELISA, IHC-P, RIA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Purified fibronectin from rat plasma

  • Positive control
    • Rat skin
  • General notes


    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Properties

Applications

Our Abpromise guarantee covers the use of ab23751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
RIA 1/5000.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN 1 antibody
    • FN 1 antibody
    • FN antibody
    • FN1 antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Transformation sensitive protein. antibody
    • Transformation sensitive protein. antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody images

  • ab23751 at 1/200 dilution staining pig fibroblast cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with antibody for 8 hours at 4°C. An Alexa-Fluor ® 488 conjugated  donkey anti-rabbit antibody was used as the secondary. The image was taken using a confocal laser scanning microscope and shows the presence of abundant fibronectin (green) in the pig fibroblasts. DAPI was used as nuclear counterstain (Blue).

    See Abreview

  • ab23751 staining rat intestine tissue section by immunohistochemistry (Formalin/PFA-fixed, paraffin-embbeded sections). Tissue underwent fixation in Bouins solution and processed by enzyme mediated antigen retrieval method. The primary antibody was diluted 1/500 and incubated with sample for 1 hour at 20°C. A HRP-conjugated mouse polyclonal to rabbit IgG was used undiluted as the secondary.

    See Abreview

  • ab23751 staining Fibronectin in Rat blood vessel tissue sections by IHC-P (formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 100% DEEB block for 10 minutes at room temperature. Enzymatic antigen retrieval was performed. Samples were incubated with primary antibody (1/1000) for 20 minutes at room temperature. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Fibronectin antibody (ab23751)

This product has been referenced in:
  • Yang HY  et al. Proteomic analysis of gingival tissue and alveolar bone during alveolar bone healing. Mol Cell Proteomics 12:2674-88 (2013). IHC ; Pig . Read more (PubMed: 23824910) »
  • Hsieh PF  et al. The role of IL-7 in renal proximal tubule epithelial cells fibrosis. Mol Immunol 50:74-82 (2012). Read more (PubMed: 22261356) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us. This product was created using Purified fibronectin from rat plasma as immunogen. As a polyclonal antibody, multiple epitope sites will be recognized throughout the target. It may be that this antibody will target solubl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Renal Proximal Tubular Epithelial cells)
Specification Renal Proximal Tubular Epithelial cells
Fixative Paraformaldehyde
Permeabilization Yes - triton x100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Username

Ms. pei-fang Hsieh

Verified customer

Submitted Jul 19 2012

Thank you for your enquiry.

I can confirm that ab23751 fibronectin antibody is tested and therefore covered by our 6 month guarantee for IHC-P and rat and pig samples. Please note that ab131065 is tested and guaranteed in IHC-P and rat, but ...

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Thank you for your enquiry.

I am sorry to confirm that ab23751 has not yet been tested in Western blot. I am pleased to hear the customer would like to make use of our testing discount program.

I can provide the following code for...

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Thank you for contacting us.  The fibronectin antibody ab23751 does indeed detect both the soluble and the insoluble form. The immunogen used to generate this antibody was fibronectin purified from plasma, i.e. the soluble fibronectin. The insolubl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (blood vessels)
Specification blood vessels
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K DAKO S3020
Permeabilization No
Blocking step DEEB DAKO S2003 as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Trachea)
Specification Trachea
Fixative Bouins
Antigen retrieval step Enzymatic
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Nov 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Intestine)
Specification Intestine
Fixative Bouins
Antigen retrieval step Enzymatic
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Nov 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Fibroblast)
Specification Fibroblast
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Mal Niladri

Verified customer

Submitted Oct 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"