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Anti-Fibronectin antibody (ab23752)

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Overview

  • Product nameAnti-Fibronectin antibodySee all Fibronectin primary antibodies ...
  • Description
    Rabbit polyclonal to Fibronectin
  • SpecificityBovine fibronectin 100%, bovine collagen type I and III <0.1%
  • Tested applicationsELISA, ICC/IF, IHC-P, RIA more details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Purified fibronectin from bovine plasma.

  • Positive controlBovine skin
  • General notes


    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Properties

Applications

Our Abpromise guarantee covers the use of ab23752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
ICC/IF ICC/IF: Use at an assay dependent dilution.
IHC-P IHC-P: Use at an assay dependent dilution.
RIA RIA: 1/10000.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P02751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody
        DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN 1 antibodyFN antibodyFN1 antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody
      see all

    References for Anti-Fibronectin antibody (ab23752)

    ab23752 has not yet been referenced specifically in any publications.

    Publishing research using ab23752 ? Please let us know so that we can cite the reference in this datasheet

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    Should I re-hydrate the sections after de-waxing and before the hyaluronidase treatment? Or should I treat with the hyaluronidase immediately after de-waxing?

    Thank you for your call today and for your patience. I have checked with my colleagues, and you should fully de-wax and re-hydrate the tissue prior to treating with the hyaluronidase. I hope this information is helpful, but please let me kn...

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    Hi, I am doing IHC-P with ab23752. I would like to know if there were any specific requirements for the hyaluronidase, such as species?

    Thanks for your inquiry. Bovine hyaluronidase will work as long as the enzyme activity is within the range given on the data sheet - "0.2% hyaluronidase (app. 300 U/mg)". I hope this helps. Please feel free to contact us if you requi...

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