Overview

  • Product nameAnti-Fibronectin antibody
    See all Fibronectin primary antibodies
  • Description
    Rabbit polyclonal to Fibronectin
  • SpecificityThis antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Only the fibronectin arch was observed in crossed immunoelectrophoresis.
  • Tested applicationsICC/IF, WB, IP, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, African Green Monkey, Chinese Hamster, Syrian Hamster
  • Immunogen

    Fibronectin isolated from a pool of normal human plasma.

  • Positive control
    • Kidney

Properties

Applications

Our Abpromise guarantee covers the use of ab2413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa).
IP Use at an assay dependent dilution. Used at 1ug/ml for 1 hr 30 min on renal carcinoma cell line 786-0 (see Abreview).
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. This antibody may be diluted to a titer of 1/50 - 1/250 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN 1 antibody
    • FN antibody
    • FN1 antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Transformation sensitive protein. antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody images

  • ICC/IF image of ab2413 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2413, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
  • ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.

  • All lanes : Anti-Fibronectin antibody (ab2413) at 1 µg/ml

    Lane 1 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 262 kDa
    Observed band size : 285 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, staining Fibronectin with ab2413.

References for Anti-Fibronectin antibody (ab2413)

This product has been referenced in:
  • Gao J  et al. Emodin suppresses hyperglycemia-induced proliferation and fibronectin expression in mesangial cells via inhibiting cFLIP. PLoS One 9:e93588 (2014). WB . Read more (PubMed: 24691542) »
  • Chiyomaru T  et al. Long non-coding RNA HOTAIR is targeted and regulated by miR-141 in human cancer cells. J Biol Chem 289:12550-65 (2014). Read more (PubMed: 24616104) »

See all 15 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rabbit Tissue lysate - other (Heart)
Specification Heart
Blocking step Super Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Aug 14 2014

The following antibodies we can recommend as per your requirements. These are tested in IHC-P and WB with mouse samples and are guaranteed.
collagen IV

http://www.abcam.com/collagen-iv-antibody-ab6586.html

anti Laminin

ht...

Read More

When transferring large proteins precipitation or incomplete transfer of the high MW protein can occur. I have included some tips about large protein transfer from our Western Blot guide below and have links this as well. I hope this is of help to you....

Read More

The doublet when using serum may be result of mild proteolysis or incomplete reduction. I would recommend making sure protease inhibitors are added to your lysis buffer directly before use and heat your samples in laemmli buffer for 20 minutes at 72C.

We have issued a free of charge replacement.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Syrian Hamster Cell lysate - whole cell (BHK cells)
Loading amount 100 µg
Specification BHK cells
Gel Running Conditions Reduced Denaturing (Tris-Glycine -10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Nov 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO cells)
Loading amount 50 µg
Specification CHO cells
Gel Running Conditions Reduced Denaturing (Tris-Glycine -10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO)
Loading amount 50 µg
Specification CHO
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Syrian Hamster Cell (BHK cells)
Specification BHK cells
Fixative Formaldehyde
Permeabilization Yes - Triton (0.1%) in PBS, 3min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Nov 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (MDCK cells)
Specification MDCK cells
Fixative Formaldehyde
Permeabilization Yes - Triton (0.1%) in PBS, 3min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Nov 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"