You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Fibronectin antibody (ab61214)

Submit an Abreview Q&A (2)

Overview

  • Product nameAnti-Fibronectin antibodySee all Fibronectin primary antibodies ...
  • Description
    Rabbit polyclonal to Fibronectin
  • SpecificityDetects endogenous levels of total Fibronectin protein
  • Tested applicationsELISA, WB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from C-terminal of human Fibronectin.

  • Positive controlExtracts from HeLa cells This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab61214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/5000.
WB WB: 1/500 - 1/1000. Detects a band of approximately 263 kDa (predicted molecular weight: 263 kDa).
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P02751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody
        DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody
      see all

    Anti-Fibronectin antibody images

    • Western blot - Fibronectin antibody (ab61214)
      Western blot - Fibronectin antibody (ab61214)
      All lanes : Anti-Fibronectin antibody (ab61214) at 1/500 dilution

      Lane 1 : extracts from HeLa cells
      Lane 2 : extracts from HeLa cells with immunizing peptide


      Predicted band size : 263 kDa
      Observed band size : 263 kDa
    • Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody (ab61214)
      Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody (ab61214)

      ICC/IF image of ab61214 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61214 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Fibronectin antibody (ab61214)

    ab61214 has not yet been referenced specifically in any publications.

    Publishing research using ab61214 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab61214 Submit a Question for ab61214

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First

    I would like to ask you about the availability of an antibody that specifically recognizes the RGD motif. After searching through your catalogue, I was only able to find references to anti-Fibronectin antibodies, but probably these, even if targeted towar...

    Read More

    Thank you for your inquiry.

    Unfortunately, you are right, we do not have any antibodies against the RGD motif alone available in our catalogue, only against the purified native, recombinant or longer peptide.


    I would like to rec...

    Read More

    I would like to know if there are any special requirements for ab61214 in WB?

    Thanks for your inquiry. Here are the recommendations for transferring large proteins efficiently during WB: The balance of SDS and methanol in the transfer buffer, protein size, and gel percentage can affect transfer efficiency. The followi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"