Anti-Fibulin 5 antibody (ab202977)
Key features and details
- Rabbit polyclonal to Fibulin 5
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Fibulin 5 antibody
See all Fibulin 5 primary antibodies -
Description
Rabbit polyclonal to Fibulin 5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Fibulin 5 aa 126-206 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9UBX5 -
Positive control
- Human placenta tissue; Human colon carcinoma lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody). |
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WB |
1/100 - 1/1000. Predicted molecular weight: 50 kDa.
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Notes |
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IHC-P
1/100 - 1/500. (or 1/50 - 1/200 if using a fluorescent secondary antibody). |
WB
1/100 - 1/1000. Predicted molecular weight: 50 kDa. |
Target
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Function
Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling. -
Tissue specificity
Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes. -
Involvement in disease
Defects in FBLN5 are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. Autosomal dominant cutis laxa is a relatively benign inherited and acquired connective tissue disorder.
Defects in FBLN5 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected.
Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3) [MIM:608895]. ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the fibulin family.
Contains 6 EGF-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10516 Human
- Entrez Gene: 23876 Mouse
- Entrez Gene: 29158 Rat
- Omim: 604580 Human
- SwissProt: Q9UBX5 Human
- SwissProt: Q9WVH9 Mouse
- SwissProt: Q9WVH8 Rat
- Unigene: 288381 Mouse
see all -
Alternative names
- ADCL2 antibody
- ARCL1A antibody
- ARMD3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Fibulin 5 with ab202977 at 1/400 dilution. Antigen retrieval was performed by boiling in sodium citrate buffer (pH 6.0) for 15 minutes, followed by endogenous peroxidase blocking with 3% hydrogen peroxide for 20 minutes. The tissue was incubated with Anti-Fibulin 5 antibody (ab202977) at 1/400 dilution overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat ovary tissue sections labeling Fibulin 5 with ab202977 at 1/500 dilution. Antigen retrieval was performed by boiling in sodium citrate buffer (pH 6.0) for 15 minutes, followed by endogenous peroxidase blocking with 3% hydrogen peroxide for 20 minutes. The tissue was incubated with Anti-Fibulin 5 antibody (ab202977) at 1/500 dilution overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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All lanes : Anti-Fibulin 5 antibody (ab202977) at 1/200 dilution
Lane 1 : Rat brain lysate
Lane 2 : Human colon carcinoma lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling Fibulin 5 with ab202977 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab202977 has been referenced in 7 publications.
- Ortega MA et al. Contribution of the Elastic Component and Venous Wall Arterialization in Patients with Venous Reflux. J Pers Med 12:N/A (2022). PubMed: 35207748
- Zheng L et al. Contribution of FBLN5 to Unstable Plaques in Carotid Atherosclerosis via mir128 and mir532-3p Based on Bioinformatics Prediction and Validation. Front Genet 13:821650 (2022). PubMed: 35356421
- Zhao B et al. Transplantation of bone marrow-derived mesenchymal stem cells with silencing of microRNA-138 relieves pelvic organ prolapse through the FBLN5/IL-1ß/elastin pathway. Aging (Albany NY) 13:3045-3059 (2021). PubMed: 33460398
- Liu CD et al. Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway. Acta Pharmacol Sin 42:370-381 (2021). PubMed: 33303991
- Yuan Y et al. A Pulmonary Vascular Model From Endothelialized Whole Organ Scaffolds. Front Bioeng Biotechnol 9:760309 (2021). PubMed: 34869270
- Peterson JC et al. Disturbed nitric oxide signalling gives rise to congenital bicuspid aortic valve and aortopathy. Dis Model Mech 13:N/A (2020). PubMed: 32801116
- Li R et al. FBLN5 is targeted by microRNA-27a-3p and suppresses tumorigenesis and progression in high-grade serous ovarian carcinoma. Oncol Rep 44:2143-2151 (2020). PubMed: 32901854