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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Anti-Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289)
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab76289 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Flow Cytometry-Filamin A antibody [EP2405Y] - Carboxyterminal end(ab76289)](/ps/datasheet/images/76/ab76289/Filamin-A-Primary-antibodies-ab76289-3.jpg)
Flow Cytometry-Filamin A antibody [EP2405Y] - Carboxyterminal end(ab76289)
Overlay histogram showing A431 cells stained with ab76289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76289 , 1/100 dilutio) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
![Western blot - Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289)](/ps/datasheet/Images/76/ab76289/ab76289_1.jpg)
Western blot - Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289)
All lanes : Anti-Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289) at 1/500000 dilution
Lane 1 : COS-1 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size : 281 kDa
Observed band size : 281 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289)](/ps/datasheet/Images/76/ab76289/ab76289_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289)
Immunohistochemical analysis of paraffin-embedded human uterus using ab76289 at a 1/100 dilution.
![Immunocytochemistry/ Immunofluorescence - Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289)](/ps/datasheet/Images/76/ab76289/ab76289_3.jpg)
Immunocytochemistry/ Immunofluorescence - Filamin A antibody [EP2405Y] - Carboxyterminal end (ab76289)
Immunofluorescent staining of HeLa cells using ab76289 at a 1/100 dilution.
![Filamin A antibody [EP2405Y] - Carboxyterminal end for WB in Mouse (76289)](/ps/datasheet/images/76/ab76289/Filamin-A-Primary-antibodies-ab76289-1.jpg)
Filamin A antibody [EP2405Y] - Carboxyterminal end for WB in Mouse (76289)
![Anti-Filamin A antibody [EP2405Y] - Carboxyterminal end for Western blot in Human (76289)](/ps/datasheet/images/76/ab76289/Filamin-A-Primary-antibodies-ab76289-14.jpg)
Anti-Filamin A antibody [EP2405Y] - Carboxyterminal end for Western blot in Human (76289)
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