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Product Name
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Filamin A antibody [FLMN01 (PM6/317)]
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See all Filamin A antibodies (10)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [FLMN01 (PM6/317)] to Filamin A
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Immunogen
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Platelet filamin.
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Reacts with
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Hu, Rat, Chk, Gpig, Rb
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Specificity
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We don't have any information regarding the epitope location of the antigen therefore we do not know which isoform(s) is recognised by this antibody.
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Western Blotting: use at a concentration of 1 - 2µg / ml for 2hrs at RT. Detects a band of approximately 250 kDa (predicted molecular weight: 250 kDa).
Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2µg / ml for 30 min at RT(requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min).
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH3T3 cells.
Skin tissue sections.
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Cellular localization
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Cytoplasm; cell cortex
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
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Relevance
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Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton: it is involved in facilitating orthogonal branching of actin filaments,linking of actin filaments to membrane glycoproteins, securing various transmembrane proteins to the actin cytoskeleton and serving as a scaffold for a wide range of cytoplasmic signaling proteins.
Filamin functions as a crosslinking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts and smooth muscle actin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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FLMN01 (PM6/317)
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Filamin A antibody [FLMN01 (PM6/317)] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Filamin A antibody [FLMN01 (PM6/317)] (ab3261)
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This product has been used in:
Jackson WM et al. Mechanical loading by fluid shear is sufficient to alter the cytoskeletal composition of osteoblastic cells. Am J Physiol Cell Physiol : (2008). PubMed: 18701650
If you publish research using ab3261 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Filamin A
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Filamin A antibody [FLMN01 (PM6/317)] - more information
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