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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Anti-Filamin A antibody (ab11074)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab11074? Please let us know so that we can cite the reference in this datasheet
ab11074 has been referenced in 3 publications.
- Castoria G et al. Androgen-induced cell migration: role of androgen receptor/filamin a association. PLoS One 6:e17218 (2011). ICC/IF; Mouse. PubMed: 21359179
- Cousin H et al. PACSIN2 regulates cell adhesion during gastrulation in Xenopus laevis. Dev Biol 319:86-99 (2008). ICC/IF, IP; Xenopus laevis. PubMed: 18495106
- Boraldi F et al. Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach. Biochim Biophys Acta 1774:1402-13 (2007). WB; Human. PubMed: 17904921
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Flow Cytometry - Filamin A antibody (ab11074)
ab11074 staining Filamin A in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated donkey anti-goat IgG (H+L) at a 1/500 dilution.
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
Immunocytochemistry/ Immunofluorescence - Filamin A antibody (ab11074)
ab11074 at a 1/2000 dilution staining Filamin A in XTC embryonic polyclonal fibroblasts by Immunocytochemistry/ Immunofluorescence.Cells were fixed with formaldehyde, permeabilized using Triton X-100 and blocked with 1% BSA. The secondary used was a Texas Red conjugated anti-goat, 1/200 dilution.
Image courtesy of Helene Cousin by Abreview.
Western blot - Anti-Filamin A antibody (ab11074)
Anti-Filamin A antibody (ab11074) at 1/6000 dilution + Human platelet lysate at 20 µg
Secondary
Rabbit anti-Goat HRP at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 250 kDa
Observed band size : >250 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
This image is courtesy of an Abreview submitted by Mahesh Shivananjappa, United States
Filamin A antibody for Flow Cytometry in Human (11074)
Filamin A antibody for WB in Xenopus laevis (11074)
Anti-Filamin A antibody for Western blot in Human (11074)
Filamin A antibody for ICC/IF in Xenopus laevis (11074)
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