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If your product does not perform as described on this datasheet, we will refund or replace your product...
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thank you very much for your technical support. I’d like to know your opinion on the reactivity obtained in my lab by using anti filamin A. Thank you very much |
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ANSWER: |
Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. Unfortunately, ab11074 has not been tested in IHC and we do not actually know if it works in this application. Reviewing this case, I would like to offer some suggestions to help optimize the results from ab11074: According to the images you send me, this antibody is working well in IHC. The staining is cytoplasmic as expected. To increase the specificity of the antibody I suggest to incubate the primary antibody at 4C over night. I can also recommend to run an experiment with more than one dilution of the antibody in parallel; and to dilute the antibody more. Unspecific staining should decrease whereas specific staining will remain in the higher dilution. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again. |
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Which chicken Filamen did you use for creating this antibody. What subtipes of filamen contained your preparate you used for immunisation?
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ANSWER: |
Thank you for your enquiry. The source used to create this antibody was chicken gizzard. This antibody is non-specific. It was not created against a specific sub-type, so it should work well on all sub-types. This is all the information I can provide you with for now. Please contact us again if you require further assistance. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
