Filamin A antibody (ab11074)

Product Name 

Filamin A antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Filamin A

Immunogen 

Full length native protein (purified) (Chicken).

Reacts with 
(species key)

Hu, Chk, Xl, Zfsh

Specificity 

Antiserum specifically stains filamin associated with stress fibers and membrane ruffles by immunofluorescent labeling of monolayer cultured cells. The product reacts best in cultured chicken fibroblasts. Good immunofluorescent labeling may also be obtained with cells of other species. In immunoblotting, antiserum specifically stains the filamin band.

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IF: 1/40.
IP: Use at an assay dependent dilution (PMID 18495106).
WB: 1/1000. Predicted molecular weight: 250 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm; cell cortex

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking

Relevance 

Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton: it is involved in facilitating orthogonal branching of actin filaments,linking of actin filaments to membrane glycoproteins, securing various transmembrane proteins to the actin cytoskeleton and serving as a scaffold for a wide range of cytoplasmic signaling proteins.

Filamin is a large flexible actin cross-linking protein present in vertebrates in both muscle and non-muscle cells. Filamin has a polypeptide molecular weight of approximately 250,000 daltons and forms dimers in solution. Filamin has been primarily purified from smooth muscle but was also identified in macrophages, neutrophils, platelets, and other cell types. Filamin cross-links actin and promotes its aggregation and gelation. Immunofluorescent labeling of a large variety of cells with Goat Anti-Filamin reveals an extensive association of the protein with the actin containing stress fibers.

Goat Anti-Filamin may be used for immunofluorescent localization of filamin in cultured cells and tissues, and to study the state of actin organization in muscle cells.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.1% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

References for Filamin A antibody (ab11074)

  This product has been used in:

Cousin H et al. PACSIN2 regulates cell adhesion during gastrulation in Xenopus laevis. Dev Biol 319:86-99 (2008). ICC/IF, IP; Xenopus laevis. PubMed: 18495106

If you publish research using ab11074 please let us know so that we can cite the reference on this datasheet.

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