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Western blot - Filamin A (phospho S2152) antibody (ab51229)
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Product Name
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Filamin A (phospho S2152) antibody
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See all Filamin A antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Filamin A (phospho S2152)
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Immunogen
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A synthetic phospho-peptide derived from human Filamin A around the phosphoicrylation site of serine 2152 (A-P-SP-V-A).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab51229 detects endogenous levels of Filamin A only when phosphorylated at serine 2152.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/300 - 1/1000. Predicted molecular weight: 281 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from 293 cells treated with EGF (200ng/ml, 5min)
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Cellular localization
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Cytoplasm; cell cortex
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
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Relevance
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Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton: it is involved in facilitating orthogonal branching of actin filaments,linking of actin filaments to membrane glycoproteins, securing various transmembrane proteins to the actin cytoskeleton and serving as a scaffold for a wide range of cytoplasmic signaling proteins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab51229 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Filamin A (phospho S2152) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Filamin A (phospho S2152) antibody images, references, products related to ab51229 and other tools.
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Filamin A (phospho S2152) antibody images:
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 Western blot - Filamin A (phospho S2152) antibody (ab51229)
All lanes : Filamin A (phospho S2152) antibody (ab51229) at 1/300 dilution
Lane 1 : 293 cell lysate stimulated with EGF (200ng/ml, 5min) with immunizing (blocking) peptide
Lane 2 : 293 cell lysate stimulated
with EGF (200ng/ml, 5min)
Predicted band size : 281 kDa
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Search PubMed (MEDLINE) for references to Filamin A
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Filamin A (phospho S2152) antibody - more information
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Customer reviews (feedback) regarding Filamin A (phospho S2152) antibody |
Customer FAQs regarding Filamin A (phospho S2152) antibody |
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Filamin A (phospho S2152) antibody - related products:
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Filamin A (phospho S2152) antibody - other tools:
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