![Western blot - Flightless I antibody [EPR4201] (ab108594) image](#Flightless-I-Primary-antibodies-ab108594-1.gif)
![Immunocytochemistry/ Immunofluorescence - Flightless I antibody [EPR4201] (ab108594) image](#Flightless-I-Primary-antibodies-ab108594-2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Flightless I antibody [EPR4201] (ab108594) image](#Flightless-I-Primary-antibodies-ab108594-4.gif)
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Product name
Anti-Flightless I antibody [EPR4201]
See all Flightless I products (6) ...
Description
Rabbit monoclonal [EPR4201] to Flightless I
Tested applications
WB, IP, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in Human Flightless I.
Positive control
HeLa, A549 and A431 cell lysates; Human skeletal muscle tissue; HeLa cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR4201
Isotype
IgG1
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Intracellular
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab108594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
IP
IP: 1/10 - 1/100.IP: 1/10 - 1/100.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF: 1/100 - 1/250.
Application notes
Is unsuitable for or Flow Cyt.
Target
Relevance
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene leads to abnormal muscle function, arrested development and embryonic lethality. The protein sequence shows that this might be a regulator of cytoskeleton and may have a role during cell division.
Cellular localization
Nucleus. Cytoplasm; cytoskeleton. Centrosome. Note: Colocalizes to actin rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
Alternative names
- Fli 1 antibody
- FLI antibody
- Fli1 antibody
see all
Database links
- Entrez Gene: 2314 Human
- Entrez Gene: 14248 Mouse
- Entrez Gene: 287375 Rat
- Omim: 600362 Human
- SwissProt: Q13045 Human
- SwissProt: Q9JJ28 Mouse
- Unigene: 513984 Human
Anti-Flightless I antibody [EPR4201] images:
Western blot - Flightless I antibody [EPR4201] (ab108594)
![Western blot - Flightless I antibody [EPR4201] (ab108594)](/ps/datasheet/images/108/ab108594/Flightless-I-Primary-antibodies-ab108594-1.gif)
All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 145 kDa
Immunocytochemistry/ Immunofluorescence - Flightless I antibody [EPR4201] (ab108594)
![Immunocytochemistry/ Immunofluorescence - Flightless I antibody [EPR4201] (ab108594)](/ps/datasheet/images/108/ab108594/Flightless-I-Primary-antibodies-ab108594-2.jpg)
ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Flightless I antibody [EPR4201] (ab108594)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Flightless I antibody [EPR4201] (ab108594)](/ps/datasheet/images/108/ab108594/Flightless-I-Primary-antibodies-ab108594-4.gif)
ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissue
References for Anti-Flightless I antibody [EPR4201] (ab108594)
ab108594 has not yet been referenced specifically in any publications.
Publishing research using ab108594? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"