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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Flotillin 1 antibody
See all Flotillin 1 products (5) ...
Description
Goat polyclonal to Flotillin 1
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Pig, Chimpanzee
Immunogen
Synthetic peptide: SISQVNHKPLRTA, corresponding to C terminal amino acids 415-427 of Human Flotillin 1.
C-SISQVNHK PLRTA
Positive control
H460 lysate
General notes
GenBank Accession Number - NP_005794
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Western blot - Flotillin 1 antibody (ab13493)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab13493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 18363778)
WB: Use a concentration of 0.2 - 1 µg/ml.Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
Target
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Sequence similarities
Belongs to the band 7/mec-2 family. Flotillin subfamily.
Cellular localization
Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionO75955
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLOT 1 antibody
- FLOT1 antibody
- FLOT1_HUMAN antibody
see all
Database links
- Entrez Gene: 10211 Human
- Omim: 606998 Human
- SwissProt: O75955 Human
- SwissProt: Q767L6 Pig
- Unigene: 179986 Human
Anti-Flotillin 1 antibody images:
Western blot - Flotillin 1 antibody (ab13493)
Anti-Flotillin 1 antibody (ab13493) at 0.3 µg/ml + H460 lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size : 49 kDa
Observed band size : 49 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
References for Anti-Flotillin 1 antibody (ab13493)
This product has been referenced in:
- He Bet al. The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88. Nat Immunol 11:836-45 (2010). ICC/IF; Human.Read more (PubMed: 20676093) »
- Masilamani Met al. Uncommon endocytic and trafficking pathway of the natural killer cell CD94/NKG2A inhibitory receptor. Traffic 9:1019-34 (2008). ICC/IF; Human.Read more (PubMed: 18363778) »
See all 4 publications for this product
Publishing research using ab13493? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"