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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Flotillin 1 antibody
See all Flotillin 1 products (5) ...
Description
Rabbit polyclonal to Flotillin 1
Tested applications
ICC, IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human
Predicted to work with
a wide range of other species
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Flotillin 1.
(Peptide available as ab41926.)
Positive control
ab41927 gave a positive result in Human Lung tissue lysate, Human Brain tissue lysate and Hela whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/200. Use with Methanol-fixed cells.
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Sequence similarities
Belongs to the band 7/mec-2 family. Flotillin subfamily.
Cellular localization
Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionO75955
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLOT 1 antibody
- FLOT1 antibody
- FLOT1_HUMAN antibody
see all
Database links
- Entrez Gene: 10211 Human
- Entrez Gene: 14251 Mouse
- Entrez Gene: 64665 Rat
- Omim: 606998 Human
- SwissProt: O75955 Human
- SwissProt: O08917 Mouse
- SwissProt: Q3TJS0 Mouse
- SwissProt: Q540I4 Mouse
see all
Anti-Flotillin 1 antibody images:
Western blot - Anti-Flotillin 1 antibody (ab41927)
All lanes : Anti-Flotillin 1 antibody (ab41927) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 47 kDa
Immunocytochemistry/ Immunofluorescence - Flotillin 1 antibody (ab41927)
ab41927 (1/200) staining Flotillin 1 in HeLa cells (green). Cells were fixed with Methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is part of an abreview submitted by Dr. Kirk McManus
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Flotillin 1 antibody (ab41927)
ab41927 (1/100) staining Flotilin 1 in paraffin embedded - Human (anal cancer), Mouse (brain) and Rat (spleen) tissues. Tissue sections underwent paraformaldehyde fixation, heat mediated antigen retrieval using Citrate Buffer pH 6.0 and were blocked using sequential peroxidase and protein block (prediluted). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
References for Anti-Flotillin 1 antibody (ab41927)
This product has been referenced in:
- Resnik Net al. Desmosome assembly and cell-cell adhesion are membrane raft-dependent processes. J Biol Chem 286:1499-507 (2011). WB, ICC/IF; Dog.Read more (PubMed: 21071449) »
- Galetto Let al. The Major Antigenic Membrane Protein of "Candidatus Phytoplasma asteris" Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors. PLoS One 6:e22571 (2011). WB, IHC-FrFl; Other.Read more (PubMed: 21799902) »
See all 4 publications for this product
Publishing research using ab41927? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"