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Anti-Flotillin 1 antibody (ab50671)

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Overview

Product name

Anti-Flotillin 1 antibody
See all Flotillin 1 products (5) ...

Description

Rabbit polyclonal to Flotillin 1

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Cow, Pig, Macaque Monkey, Rhesus monkey, Orangutan

Immunogen

Synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in Rat and differs by one amino acid in Human

GVSISQVNHK PLRTA

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human HEK-293T cells and whole cell extract of mouse/rat lung.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

  • Immunocytochemistry/ Immunofluorescence - Flotillin 1 antibody (ab50671)Immunocytochemistry/ Immunofluorescence - Flotillin 1 antibody (ab50671) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab50671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at a concentration of 5 - 10 µg/ml (recommended with Human HEK-293T cells).
WB: Use at a concentration of 0.5 - 2.0 µg/ml (recommended with whole cell extract of mouse/rat lung). Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Sequence similarities

Belongs to the band 7/mec-2 family. Flotillin subfamily.

Cellular localization

Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accessionO75955 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • FLOT 1 antibody
  • FLOT1 antibody
  • FLOT1_HUMAN antibody
  • Flotillin-1 antibody
  • Flotillin1 antibody
  • Integral membrane component of caveolae antibody
  • Reggie 2 antibody
see all

Database links

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Anti-Flotillin 1 antibody images:

  Immunocytochemistry/ Immunofluorescence - Flotillin 1 antibody (ab50671)

Immunocytochemistry/ Immunofluorescence - Flotillin 1 antibody (ab50671)

Indirect Immunofluorescence. Human 293T cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 10 µg/mL Anti-Flotillin 1. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.

References for Anti-Flotillin 1 antibody (ab50671)

This product has been referenced in:

  • Albrechtsen Ret al. Extracellular engagement of ADAM12 induces clusters of invadopodia with localized ectodomain shedding activity. Exp Cell Res 317:195-209 (2011).Read more (PubMed: 20951132) »
  • Sandgren KJet al. A differential role for macropinocytosis in mediating entry of the two forms of vaccinia virus into dendritic cells. PLoS Pathog 6:e1000866 (2010). ICC/IF; Human.Read more (PubMed: 20421949) »

See all 2 publications for this product

Publishing research using ab50671? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"