Anti-Flotillin 2/ESA antibody (ab96507)
Key features and details
- Rabbit polyclonal to Flotillin 2/ESA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Flotillin 2/ESA antibody
See all Flotillin 2/ESA primary antibodies -
Description
Rabbit polyclonal to Flotillin 2/ESA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Flotillin 2/ESA aa 365-428 (C terminal).
Database link: Q14254 -
Positive control
- WB: HeLa. Mouse brain lysate. Rat brain lysate. IP: Human HeLa whole cell lysate. IHC-P: human lung cancer. ICC/IF: HeLa S3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
1/100 - 1/1000.
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IP | (1) |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
Target
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Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. -
Tissue specificity
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. -
Sequence similarities
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
Cellular localization
Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 2319 Human
- Entrez Gene: 14252 Mouse
- Entrez Gene: 83764 Rat
- Omim: 131560 Human
- SwissProt: Q14254 Human
- SwissProt: Q60634 Mouse
- SwissProt: Q9Z2S9 Rat
- Unigene: 514038 Human
see all -
Alternative names
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human lung cancer using ab96507 at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
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Flotillin 2 was immunoprecipitated from 5µ of HeLa whole cell lysate with ab96507. Western blot was performed from the immunoprecipitate using ab96507. EasyBlot anti-Rabbit IgG, was used as secondary antibody.
Lane 1: HeLa lysate 5µg (Input).
Lane 2: ab96507 IP in HeLa lysate. -
Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Rat brain lysate at 50 µg
Predicted band size: 47 kDa10% SDS-PAGE
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Flotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.
Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
Lane 3 : Input (HeLa cell lysate)
Lane 4 : Supernatant IP (using non-Abcam antibody)
Lane 5 : Supernatant IP (using ab96507)
Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody -
Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab96507 has been referenced in 6 publications.
- Biernatowska A et al. MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules. Cells 11:N/A (2022). PubMed: 35159121
- Bai C et al. Cryopreservation disrupts lipid rafts and heat shock proteins in yellow catfish sperm. Cryobiology 87:32-39 (2019). PubMed: 30876909
- Li T et al. FLOT2 overexpression is associated with the progression and prognosis of human colorectal cancer. Oncol Lett 17:2802-2808 (2019). PubMed: 30854055
- Gong J et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). PubMed: 27354374
- Zhu Q et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . PubMed: 26443540
- Wang X et al. Flotillin-2 is associated with breast cancer progression and poor survival outcomes. J Transl Med 11:190 (2013). WB . PubMed: 23945257