Flt 3 Ligand Mouse ELISA Kit (ab113342)
- Product nameFlt 3 Ligand Mouse ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 15 pg/ml
- Range20 pg/ml - 5000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.49 67% - 103% Serum 91.81 83% - 99% Plasma 77.99 69% - 85%
- Species reactivityReacts with: Mouse
- Product overview
ab113342 Mouse Flt 3 Ligand (chemokine, interferon gamma-inducible T cell a chemoattractant) ELISA(Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Flt 3 Ligand in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse Flt 3 Ligand coated on a 96- well plate. Standards and samples are pipetted into the wells and Flt 3 Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Flt 3 Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt 3 Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A Item D 1 x 30ml Assay Diluent B 5x conc Item E 1 x 15ml Detection Antibody Flt 3 Ligand Item F 2 vials Flt 3 Ligand Microplate (12 strips x 8 wells) coated with anti-mouse Flt 3 Ligand 1 unit Flt 3 Ligand Standard Mouse (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- Flt 3 ligandFlt 3LFlt3 L
- FLT3 LGFlt3 ligandFlt3LFLT3L_HUMANFlt3lgFms related tyrosine kinase 3 ligandFms-related tyrosine kinase 3 ligandSL cytokine
- SwissProt: P49772 Mouse
Our Abpromise guarantee covers the use of ab113342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Flt 3 Ligand Mouse ELISA Kit (ab113342)
ab113342 has not yet been referenced specifically in any publications.