Flt3 / CD135 protein (Active) (ab83998)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab83998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Molecular Mass: ab83998 migrates as a broad band between 100 and 130 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 85 kDa.
pI: ab83998 separates into a number of isoforms with a pI between 5.1 and 6.8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This
compares with the unmodified protein that has a predicted pI of 6.43.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- CD 135CD135CD135 antigen
- Fetal liver kinase 2FL cytokine receptorFlk 2Flk2Flt 3FLT-3Flt3FLT3_HUMANFMS like tyrosine kinase 3Fms related tyrosine kinase 3Fms-like tyrosine kinase 3Growth factor receptor tyrosine kinase type IIILy-72OTTHUMP0000004234Receptor type tyrosine protein kinase FLT3Stem cell tyrosine kinase 1Stk 1STK-1Stk1Tyrosine protein kinase receptor FLT3Tyrosine-protein kinase receptor FLT3
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain.
Flt3 / CD135 protein (Active) images
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83998 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83998
Lane 2: ab83998 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83998 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 µg protein loaded per lane; Deep Purple™ stained.
A sample of ab83998 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein loaded. Spot train indicates presence of multiple isoforms.
References for Flt3 / CD135 protein (Active) (ab83998)
ab83998 has not yet been referenced specifically in any publications.