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Overview

  • Product nameFlt3 / CD135 protein (Active)See all Flt3 / CD135 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-540 of Human Flt3 / CD135 fused to the Fc region of Human IgG1 (aa 93-330) expressed in modified Human 293 cells. Swiss ID = P36888.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityActivity: The ED50 of ab83998 is typically 1.0 -2.0 ng/ml as measured by its ability to neutralize Flt3 mediated proliferation of the OCI/AML5 human leukemia cell line.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: NQDLPVIKCVLINHKNNDSSVGKSSSYPM VSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVL VDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKM TETQAGEYLLFIQSEATNYTILFTVSIRNTL LYTLRRP YFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESP AVVKKEEKVLHELFGTDIRCC ARNELGRECTRLFTIDL NQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELEN KALEEGNYFEM STYSTNRTMIRILFAFVSSVARNDTGY YTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEF CFS VRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISK FCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRK PQVLA EASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVW NRKANRKVFGQWVSSSTLNM SEAIKGFLVKCCAYNSLG TSCETILLNSPGPFPFIQDRIPKVDKKVEPKSCDKTHTCP PCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV L TVLHQDWLNGKEYKCRVSNKALPAPIEKTISKAKGQP REPQVYTLPPSRDELTKNQVSLTCLVKGFYP SDIAVEW ESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGN VFSCSVMHEALHNHYTQKS LSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83998 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab83998 migrates as a broad band between 100 and 130 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 85 kDa.

    pI: ab83998 separates into a number of isoforms with a pI between 5.1 and 6.8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This
    compares with the unmodified protein that has a predicted pI of 6.43.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD 135CD135CD135 antigen
        Fetal liver kinase 2FL cytokine receptorFlk 2Flk2Flt 3FLT-3Flt3FLT3_HUMANFMS like tyrosine kinase 3Fms related tyrosine kinase 3Fms-like tyrosine kinase 3Growth factor receptor tyrosine kinase type IIILy-72OTTHUMP0000004234Receptor type tyrosine protein kinase FLT3Stem cell tyrosine kinase 1Stk 1STK-1Stk1Tyrosine protein kinase receptor FLT3Tyrosine-protein kinase receptor FLT3
      see all
  • FunctionReceptor for the FL cytokine. Has a tyrosine-protein kinase activity.
  • Tissue specificityBone marrow cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P36888 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Flt3 / CD135 protein (Active) images

    • Functional Studies - Flt3 / CD135 protein (Fc Chimera Active) (ab83998)
      Functional Studies - Flt3 / CD135 protein (Fc Chimera Active) (ab83998)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - Flt3 / CD135 protein (Fc Chimera Active) (ab83998)
      SDS-PAGE - Flt3 / CD135 protein (Fc Chimera Active) (ab83998)
      1D SDS-PAGE of ab83998 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab83998
      Lane 2: ab83998 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab83998 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
      10 µg protein loaded per lane; Deep Purple™ stained.
    • SDS-PAGE - Flt3 / CD135 protein (Fc Chimera Active) (ab83998)
      SDS-PAGE - Flt3 / CD135 protein (Fc Chimera Active) (ab83998)
      A sample of ab83998 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein loaded. Spot train indicates presence of multiple isoforms.

    References for Flt3 / CD135 protein (Active) (ab83998)

    ab83998 has not yet been referenced specifically in any publications.

    Publishing research using ab83998 ? Please let us know so that we can cite the reference in this datasheet

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