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Overview

  • Product nameFlt3 / CD135 protein (Active)See all Flt3 / CD135 proteins and peptides ...
  • Protein descriptionRecombinant fragment of human Flt3 (aa 564-end) with an N-terminal His tag. Predicted Mw = 54.2kDa.
  • Molecular weight54.200kDa

    • Properties

  • Purification notes>80% by SDS-PAGE.
  • Biological activityActivity: 20 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30 C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant Flt3.
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
  • Concentration information loading...
  • Additional notesThis protein was expressed in a Baculovirus infected Sf9 cell expression system. Expected molecular weight of 54.2 kDa.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab42610 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Inhibition Assay
  • Application notesInhib: Use at an assay dependent dilution.

    Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD 135CD135CD135 antigen
        Fetal liver kinase 2FL cytokine receptorFlk 2Flk2Flt 3FLT-3Flt3FLT3_HUMANFMS like tyrosine kinase 3Fms related tyrosine kinase 3Fms-like tyrosine kinase 3Growth factor receptor tyrosine kinase type IIILy-72OTTHUMP0000004234Receptor type tyrosine protein kinase FLT3Stem cell tyrosine kinase 1Stk 1STK-1Stk1Tyrosine protein kinase receptor FLT3Tyrosine-protein kinase receptor FLT3
      see all
  • FunctionReceptor for the FL cytokine. Has a tyrosine-protein kinase activity.
  • Tissue specificityBone marrow cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P36888 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Flt3 / CD135 protein (Active) (ab42610)

    ab42610 has not yet been referenced specifically in any publications.

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