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Overview

  • Product nameFlt3 ligand Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1.5 pg/ml
  • Range
    4.1 pg/ml - 400 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.62 85% - 108%
    Serum 95.48 84% - 107%
    Plasma 94.39 83% - 106%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab10521 Flt3 ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Flt3 ligand in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Flt3 ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman Flt3 ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsELISA more details

Properties

  • RelevanceFlt3 ligand is a ligand for the FLT3 tyrosine kinase receptor and belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3 ligand have been identified. The predominant form is the transmembrane form, which is biologically active on the cell surface. When proteolytically cleaved the transmembrane isoform generates a soluble form, which is also biologically active. Flt3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3 ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation.
    • Alternative names
        Flt 3LFlt3 LFLT3 LG
        Flt3LFLT3LG Fms related tyrosine kinase 3 ligand SL cytokine
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100521 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Flt3 ligand Human ELISA Kit images

      • ELISA - Flt3 ligand Human ELISA Kit (ab100521)
        ELISA - Flt3 ligand Human ELISA Kit (ab100521)

      Protocols

      References for Flt3 ligand Human ELISA Kit (ab100521)

      ab100521 has not yet been referenced specifically in any publications.

      Publishing research using ab100521 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab100521 Submit a Question for ab100521

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      10
      Votes: Highest First

      The sample tested were supernatants (IMDM+30%FBS and StemSpan serum free medium) which have been in contact with Mononuclear cells.

      - I didn’t dilute the samples

      - The only step altered was the overnight incubation at 4C (step 2) w...

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      Thank you very much for sending the details. These were very helpful.

      As we discussed on phone please try the experiment again by keeping all reagents at room temperature.

      Please let me know if you need further assistance.

      ELISA Kit: Why there are 2 vilas of secondary antibody?

      Thank you very much for your phone call this morning.

      As discussed I will be followingthis case further. Could you please provide the following details;

      - Types of sample used. If supernatant, what type of cells were incubated
      - Dilu...

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