Flt3 ligand Human ELISA Kit (ab100521)
- Product nameFlt3 ligand Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 1.5 pg/ml
- Range4.1 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.62 85% - 108% Serum 95.48 84% - 107% Plasma 94.39 83% - 106%
- Species reactivityReacts with: Human
- Product overview
ab10521 Flt3 ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Flt3 ligand in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Flt3 ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman Flt3 ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 600X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody Flt-3L: biotinylated anti-human Flt-3L 2 vials Flt 3L Standard Human (recombinant) 2 vials Flt-3L Microplate (12 strips x 8 wells) coated with anti-human Flt-3L 1 unit HRP-Streptavidin Concentrate 600x concentrated HRP-conjugated streptavidin 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Flt 3LFlt3 LFLT3 LG
- Flt3LFLT3LG Fms related tyrosine kinase 3 ligand SL cytokine
- SwissProt: P49771 Human
Our Abpromise guarantee covers the use of ab100521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Flt3 ligand Human ELISA Kit (ab100521)
ab100521 has not yet been referenced specifically in any publications.