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Flt3 ligand protein (Active) (ab9690)

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Overview

  • Product nameFlt3 ligand protein (Active)See all Flt3 ligand proteins and peptides ...
  • Protein descriptionHighly pure (>98%) recombinant human Flt3 ligand.
  • Expression hostE. coli

    • Properties

  • Purification notesSterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised:Reconstitute with distilled water to achieve desired concentration.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
  • Concentration information loading...
  • Additional notesThe ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
  • Sequence notesRecombinant human flt3-ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues, which comprises the extracellular domain of the transmembrane flt3-ligand protein: TQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMERLKTVAGS KMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLL QETS EQLVALKPWITRQNFSRCLE LQCQPDSSTL PPP WSPRPLE ATAPTA
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab9690 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab9688.

    • Protein info

    • Alternative names
        Flt 3 ligandFlt 3LFlt3 L
        FLT3 LGFlt3 ligandFlt3LFLT3L_HUMANFlt3lgFms related tyrosine kinase 3 ligandFms-related tyrosine kinase 3 ligandSL cytokine
      see all
  • FunctionStimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P49771 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Flt3 ligand protein (Active) (ab9690)

    ab9690 has not yet been referenced specifically in any publications.

    Publishing research using ab9690 ? Please let us know so that we can cite the reference in this datasheet

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    Good morning I've bought FLT3-ligand (Ab9690) but in the data sheet there is not suggestion about the reconstitution of the lyophilized protein. What is the best buffer? and the best concentration? Lot: 49746

    Thank you for your enquiry. The reconstitution instructions for this lot. Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"