Follicle Stimulating Hormone Human ELISA Kit (ab108641)

Overview

  • Product nameFollicle Stimulating Hormone Human ELISA KitSee all Follicle Stimulating Hormone kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 2.5 mIU/ml
  • Range
    2.5 mIU/ml - 200 mIU/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Follicle Stimulating Hormone in vitroELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Follicle Stimulating Hormone in Human serum.

    A 96-well plate has been precoated with anti-Follicle Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Follicle Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Follicle Stimulating Hormone HRP conjugate binds to the antibody-Follicle Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Follicle Stimulating Hormone in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Alternative names
    • Follicle-stimulating hormone
    • Follitropin
    • FSH
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Follicle Stimulating Hormone Human ELISA Kit images

  • Representative standard curve using ab108641

Protocols

References for Follicle Stimulating Hormone Human ELISA Kit (ab108641)

ab108641 has not yet been referenced specifically in any publications.

Product Wall

I have been informed by the lab that while these are specific to human FSH, the mouse serum may cause some background. We recommend optimizing the tests by running a serial dilution of the mouse serum to determine which dilution factor gives the least...

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TheFollicle Stimulating Hormone Human ELISA Kit (ab108641) uses and anti-alpha-FSH monoclonal antibody for the capture and an anti-beta-FSH monoclonal antibody for the detection. ...

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We do have no ELISA kit against LH, FSH or Progesterone which are tested against the rat proteins. We do have however such kits tested against the human protein, for which we have however not yet tested w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"