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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Abcam’s Follicle Stimulating Hormone in vitroELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Follicle Stimulating Hormone in Human serum.
A 96-well plate has been precoated with anti-Follicle Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Follicle Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Follicle Stimulating Hormone HRP conjugate binds to the antibody-Follicle Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Follicle Stimulating Hormone in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Follicle Stimulating Hormone Coated Microplate (12 x 8 wells)||1 unit|
|Anti-Follicle Stimulating Hormone HRP Conjugate||1 x 13ml|
|Follicle Stimulating Hormone Standard 0 - 0 mU/mL (Lyophilized)||1 vial|
|Follicle Stimulating Hormone Standard 1 - 5 mU/mL (Lyophilized)||1 vial|
|Follicle Stimulating Hormone Standard 2 - 15 mU/mL (Lyophilized)||1 vial|
|Follicle Stimulating Hormone Standard 3 - 50 mU/mL (Lyophilized)||1 vial|
|Follicle Stimulating Hormone Standard 4 - 100 mU/mL (Lyophilized)||1 vial|
|Follicle Stimulating Hormone Standard 5 - 200 mU/mL (Lyophilized)||1 vial|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab108641 has not yet been referenced specifically in any publications.
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