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Overview

  • Product nameFormate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.

  • Tested applicationsFunctional Studies more details

Properties

Applications

Our Abpromise guarantee covers the use of ab111748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies FuncS

Formate Assay Kit images

  • Functional Studies - Formate Assay Kit (ab111748)
    Functional Studies - Formate Assay Kit (ab111748)
    Example of standard curve obtained using ab111748

Protocols

References for Formate Assay Kit (ab111748)

ab111748 has not yet been referenced specifically in any publications.

Publishing research using ab111748 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab111748 Submit a Question for ab111748

Displaying 1 - 2 of 2 results for Abreviews and Q&A

10
Votes: Highest First

I was wondering what the assay buffer of the kit contains.

We are concerned with the activity of native formate dehydrogenase in the homogenous samples.

Is there any way to inactivate those enzymes without disturbing the endogenous f...

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Thank you for contacting Abcam

The recipe for the Formate assay buffer is proprietary, but it does contain some NP-40 (< 0.5 %).

Please let me know if you have any other questions.

To Abcam technical support,

I am using the kit to measure formate level in cyanobacteria. Cyanobacteria is gram-negative, but their peptidoglycan layer is thicker, and they also have cell walls.

My question is, what homogenization pr...

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Thank you for your enquiry.

I agree that perhaps a mechanical extraction method would be best for this assay. Here is a link to a publication that discusses a variety of different methods to produce cell extracts from cyanobacteria.

ht...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"