Products:Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
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ab15737 |
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ab15592 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Fragilis antibody - Primordial Germ Cell Marker (ab15592) at 1 µg/ml
Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Stem Cell lysate with
Lane 4 : Mouse Embryonic Germ Cell lysate with
Lysates/proteins at 10 µg per lane.
Predicted band size : 15 kDa
Observed band size : 13 kDa (why is the actual band size different from the predicted?)
IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).
ab15592 (anti-Fragilis) = red
Oct4 = green
DAPI (DNA) = blue
The dilution of ab15592 used was 10 ug/ml.
Petra Hajkova, University of Cambridge
Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
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