You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Fragilis antibody - Primordial Germ Cell Marker (ab15592)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Fragilis antibody - Primordial Germ Cell Marker
See all Fragilis products (5) ...

Description

Rabbit polyclonal to Fragilis - Primordial Germ Cell Marker

Tested applications

IHC-P, ICC/IF, IHC-Fr, WBmore details

Cross reactivity

Reacts with

Mouse

Predicted to work with

Rat

Immunogen

Synthetic peptide derived from residues 1 - 50 of Mouse Fragilis.

(Peptide available as ab15737.)

Positive control

WB: Mouse Embryonic Stem Cell lysate, Mouse Embryonic Germ Cell lysate IHC-Fr: male genital ridges of E12.5 mouse embryos

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Fragilis antibody - Germ Line Marker (ab15592)Western blot - Fragilis antibody - Germ Line Marker (ab15592) image (enlarge)

  • Immunohistochemistry - Fragilis antibody - Germ Line Marker (ab15592)Immunohistochemistry - Fragilis antibody - Germ Line Marker (ab15592) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Fragilis antibody - Primordial Germ Cell Marker (ab15592)Immunocytochemistry/ Immunofluorescence - Fragilis antibody - Primordial Germ Cell Marker (ab15592) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab15592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.

Sequence similarities

Belongs to the CD225 family.

Post-translational
modifications

Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionQ01628 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 1 8U antibody
  • Ifitm 3 antibody
  • Ifitm3 antibody
  • IFM3_HUMAN antibody
  • Interferon induced transmembrane protein 3 (1 8U) antibody
  • Interferon Induced Transmembrane Protein 3 antibody
  • Interferon inducible antibody
  • Interferon inducible protein 1 8U antibody
  • Interferon Inducible Protein 15 antibody
  • Interferon Inducible Protein Homolog antibody
  • Interferon-induced transmembrane protein 3 antibody
  • Interferon-inducible protein 1-8U antibody
  • IP15 antibody
see all

Anti-Fragilis antibody - Primordial Germ Cell Marker images:

  Western blot - Fragilis antibody - Germ Line Marker (ab15592)

Western blot - Fragilis antibody - Germ Line Marker (ab15592)

All lanes : Anti-Fragilis antibody - Primordial Germ Cell Marker (ab15592) at 1 µg/ml

Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml

Lysates/proteins at 10 µg per lane.


Predicted band size : 15 kDa
Observed band size : 13 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry - Fragilis antibody - Germ Line Marker (ab15592)

Immunohistochemistry - Fragilis antibody - Germ Line Marker (ab15592)

IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).

ab15592 (anti-Fragilis) = red
Oct4 = green
DAPI (DNA) = blue

The dilution of ab15592 used was 10 ug/ml.

Petra Hajkova, University of Cambridge

  Immunocytochemistry/ Immunofluorescence - Fragilis antibody - Primordial Germ Cell Marker (ab15592)

Immunocytochemistry/ Immunofluorescence - Fragilis antibody - Primordial Germ Cell Marker (ab15592)

Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.

References for Anti-Fragilis antibody - Primordial Germ Cell Marker (ab15592)

This product has been referenced in:

  • Zou Ket al. Improved Efficiency of Female Germline Stem Cell Purification Using Fragilis-Based Magnetic Bead Sorting. Stem Cells Dev : (2011). ICC/IF, IHC-P, Purification; Mouse.Read more (PubMed: 21615296) »
  • Feeley EMet al. IFITM3 Inhibits Influenza A Virus Infection by Preventing Cytosolic Entry. PLoS Pathog 7:e1002337 (2011). WB, ICC/IF; Human.Read more (PubMed: 22046135) »

See all 3 publications for this product

Publishing research using ab15592? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"