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Products:Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Overview
Product name
Anti-Fragilis antibody - Primordial Germ Cell Marker
See all Fragilis products (5) ...
Description
Rabbit polyclonal to Fragilis - Primordial Germ Cell Marker
Tested applications
IHC-P, ICC/IF, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat
Immunogen
Synthetic peptide derived from residues 1 - 50 of Mouse Fragilis.
(Peptide available as ab15737.)
Positive control
WB: Mouse Embryonic Stem Cell lysate, Mouse Embryonic Germ Cell lysate IHC-Fr: male genital ridges of E12.5 mouse embryos
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration. (PubMed: 21615296)
ICC/IF: Use a concentration of 5 µg/ml
IHC-Fr: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).Can be blocked with Fragilis peptide (ab15737).
Target
Function
IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.
Sequence similarities
Belongs to the CD225 family.
Post-translational
modifications
Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ01628
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 8U antibody
- Ifitm 3 antibody
- Ifitm3 antibody
see all
Database links
- Entrez Gene: 66141 Mouse
- Entrez Gene: 361673 Rat
- SwissProt: Q9CQW9 Mouse
- SwissProt: P26376 Rat
- Unigene: 141021 Mouse
- Unigene: 145191 Rat
Anti-Fragilis antibody - Primordial Germ Cell Marker images:
Western blot - Fragilis antibody - Germ Line Marker (ab15592)
All lanes : Anti-Fragilis antibody - Primordial Germ Cell Marker (ab15592) at 1 µg/ml
Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Predicted band size : 15 kDa
Observed band size : 13 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry - Fragilis antibody - Germ Line Marker (ab15592)
IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).
ab15592 (anti-Fragilis) = red
Oct4 = green
DAPI (DNA) = blue
The dilution of ab15592 used was 10 ug/ml.
Petra Hajkova, University of Cambridge
Immunocytochemistry/ Immunofluorescence - Fragilis antibody - Primordial Germ Cell Marker (ab15592)
Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
References for Anti-Fragilis antibody - Primordial Germ Cell Marker (ab15592)
This product has been referenced in:
- Zou Ket al. Improved Efficiency of Female Germline Stem Cell Purification Using Fragilis-Based Magnetic Bead Sorting. Stem Cells Dev : (2011). ICC/IF, IHC-P, Purification; Mouse.Read more (PubMed: 21615296) »
- Feeley EMet al. IFITM3 Inhibits Influenza A Virus Infection by Preventing Cytosolic Entry. PLoS Pathog 7:e1002337 (2011). WB, ICC/IF; Human.Read more (PubMed: 22046135) »
See all 3 publications for this product
Publishing research using ab15592? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"