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Product name
Frataxin ELISA Kit
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 4 | 9.9% |
Tests
1 x 96 well plate
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Quantitative
Sensitivity
0.3 ng/ml
Recovery
107%
| Sample type | Average % | Range % |
|---|---|---|
| Cell culture supernatant | 107 | 86 - 118 |
| Serum | 107 | 99 - 111 |
Cross reactivity
Reacts with
Mouse, Rat, Cow, Dog, Human
Product overview
ab115346 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Frataxin in cell and tissue lysates. The assay employs an antibody specific for Frataxin coated on a 96-well plate. Standards and samples are pipetted into the wells and Frataxin present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-Frataxin detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Frataxin bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Store all components at 4°C. This kit is stable for 6 months from
receipt. After reconstitution the standard should be stored at -80°C.
receipt. After reconstitution the standard should be stored at -80°C.
Tested applications
ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 10X Frataxin protein Detector Antibody | 1 x 0.7ml | |
| 10X HRP Label | 1 x 1ml | |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| Human mature frataxin protein standard | 1 vial | |
| Microplate 96 antibody coated wells in 12 strips | 1 unit | |
| TMB Development Solution | P/N 8209803 | 1 x 12ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Ion and metal assay kits
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Vitamins / Minerals
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Mitochondrial
Alternative names
- MS956
Applications
Show applications key
Our Abpromise guarantee covers the use of ab115346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Frataxin ELISA Kit images:
ELISA - Frataxin ELISA Kit (ab115346)
An experiment to determine the levels of frataxin in human samples. Transformed B lymphocyte cells from Friedreich's Ataxia samples, due to a stable homozygous GAA repeat insertion, are compared to heterozygous carrier B-lymphocyte cells and control B-lymphocyte cells. A standard curve was created as described in the protocol. B lymphocyte extracts were analyzed at 0.2 mg/mL (10µg/well) and frataxin concentrations were interpolated from the standard curve.
Protocols
References for Frataxin ELISA Kit (ab115346)
ab115346 has not yet been referenced specifically in any publications.
Publishing research using ab115346? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"