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ab109881 is used to rapidly quantify frataxin protein levels from human sample materials. Purification of mitochondria is not necessary for the performance of this assay. Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the mature form of frataxin. One antibody is immobilized on the nitrocellulose membrane of the dipstick in a thin line perpendicular to the length of the dipstick while the other is gold-conjugated which gives a visual signal. When frataxin is present in the sample, a red-colored line appears on the dipstick at the site of the anti-frataxin mAb immobilized on the membrane. The signal intensity is directly related to the level of frataxin in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.
All components are stable in their provided containers at room temperature out of direct sunlight.
After diluting the 10X Blocking Buffer to 2X, store at 4°C.
For long-term storage, all buffers can be stored at 4°C.
|Components||90 tests||30 tests|
|Buffer B (10X Blocking solution)||3 x 0.4ml||1 x 0.4ml|
|Dipsticks||1 x 90 units||1 x 30 units|
|Extraction Buffer||3 x 15ml||1 x 15ml|
|Gold-conjugated antibody (dried in microplate wells)||1 x 90 tests||1 x 30 tests|
|Wash buffer||3 x 1.5ml||1 x 1.5ml|
Our Abpromise guarantee covers the use of ab109881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|