MitoSciences (MSF31-30)

Frataxin Protein Quantity Dipstick Assay Kit (ab109881)

Overview

  • Product nameFrataxin Protein Quantity Dipstick Assay Kit
    See all Frataxin kits
  • Sample type
    Whole Blood
  • Assay typeSandwich (quantitative)
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab109881 is used to rapidly quantify frataxin protein levels from human sample materials. Purification of mitochondria is not necessary for the performance of this assay. Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the mature form of frataxin. One antibody is immobilized on the nitrocellulose membrane of the dipstick in a thin line perpendicular to the length of the dipstick while the other is gold-conjugated which gives a visual signal. When frataxin is present in the sample, a red-colored line appears on the dipstick at the site of the anti-frataxin mAb immobilized on the membrane. The signal intensity is directly related to the level of frataxin in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.

  • Notes

    All components are stable in their provided containers at room temperature out of direct sunlight.

    After diluting the 10X Blocking Buffer to 2X, store at 4°C.

    For long-term storage, all buffers can be stored at 4°C.

  • Tested applicationsSandwich ELISAmore details
  • PlatformReagents

Properties

Applications

Our Abpromise guarantee covers the use of ab109881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Frataxin Protein Quantity Dipstick Assay Kit images

  • An example using ab109881 to quantify frataxin levels from β -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients. Shown is a 1:2 dilution series using a positive control sample. Approximately 6 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 4 µg of control sample. A one-site hyperbola line was generated for best-fit analysis using GraphPad.
  • An example using ab109881 to quantify frataxin levels from β -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients. Based on the above standard curve values, of protein extract for the controls and FA patient samples. (Note: for a statistical analysis, it is preferred to use two dipsticks for each sample; intra-assay CV's are typically <=10%.) For this analysis, the FA patients had between 550 - 925 GAA repeats on the smaller allele. Using GraphPad software, the signal intensity from the standard curve was interpolated and the relative amount of frataxin in the patients, as compared to the controls, was determined. Based on the above analysis, the patient samples had between 13% and 20% of frataxin levels compared to the control.
  • Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.

Protocols

References for Frataxin Protein Quantity Dipstick Assay Kit (ab109881)

This product has been referenced in:
  • Deutsch EC  et al. Usefulness of frataxin immunoassays for the diagnosis of Friedreich ataxia. J Neurol Neurosurg Psychiatry N/A:N/A (2014). Human . Read more (PubMed: 24463479) »
  • Sandi C  et al. Generation and characterisation of Friedreich ataxia YG8R mouse fibroblast and neural stem cell models. PLoS One 9:e89488 (2014). Read more (PubMed: 24586819) »

See all 12 Publications for this product

Product Wall

Thank you for this information.

The control lines are not all the same between kits. Within a single kit the control can also change –it’s possible if the signal from the sample is very strong this can deplete the mount gold labe...

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We have done pretty extensive testing with the antibodies used in MSF31 to show that is human specific - the detector antibody is human only.

So If there is a band in mouse I would be concerned about ...

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No the antibodies in these two kits are not the same- dipsticks ab109881 contains antibodies that are human specific these are the same human specific antibodies can be found in ELISA kit ab110173.

Howeve...

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Thank you for contacting us yesterday in regards to the Frataxin and Pyruvate dehydrogenase Quantity Dipstick kits (ab109881 and ab109883 respectively).

As discussed over the phone, I contacted MitoSciences to discuss if the kits would be com...

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Thank you for your enquiry.

For the sandwich ELISA dipstick ab109881, the buffers are the same. However for the PDH activity dipstick ab109883 they are very different.

I am sorry that as we have over 80 0000 products it is not pos...

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Please find below the answers from my colleagues from Abcam-MitoSciences regarding the kit ab109881:

1)This is normal. The detergent provided with the kit is a very mild detergent and which does not solubi...

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I assume the samples you are using are just recombinant human Frataxin, not a cell lysate?

To increase the signal it is suggested to add more sample to another dipstick.

What type of dipstick ...

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This is the information, we received from the lab:

The exact number for ab109881 will vary from batch to batch of kit and there will be a small variation depending on how old the kit is. However, roughl...

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Thank you for your enquiry.

I am pleased to help. I assume you are referring to the following frataxin kits from our catalog:

ab109881 Frataxin Protein Quantity Dipstick Assay Kit
Reacts with: Human
http://www.abcam.com/...

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Thank you for your calls this week and for your patience.

I attempted to return your call today but the number that I have in my notes is still not correct. Could you send me the number where I can reach you?

I'm still in the proces...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"