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Overview

  • Product nameFree Glycerol Assay KitSee all Glycerol kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 10000 µM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.
    • Alternative names
        GlycerinGlycerine

    Applications

    Our Abpromise guarantee covers the use of ab65337 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Free Glycerol Assay Kit images

    • Functional Studies - Free Glycerol Assay Kit (ab65337)
      Functional Studies - Free Glycerol Assay Kit (ab65337)
      Glycerol Standard Curve using ab65337.

    Protocols

    References for Free Glycerol Assay Kit (ab65337)

    This product has been referenced in:
    • Carley AN  et al. Multiphasic triacylglycerol dynamics in the intact heart during acute in vivo overexpression of CD36. J Lipid Res 54:97-106 (2013). Functional Studies . Read more (PubMed: 23099442) »

    See 1 Publication for this product

    Publishing research using ab65337 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65337 Submit a Question for ab65337

    Displaying 1 - 10 of 10 results for Abreviews and Q&A

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    Votes: Highest First

    Can I use the same method of samples preparation in the protocol of Triglyceride Quantification Kit to prepare the samples to use your Free Glycerol Assay Kit to measure intracellular glycerol?

    No, I don’t think preparing samples once for both assays is a good idea. For the glycerol assay, you just need cell homogenates, whereas for the triglyceride assay you are actually preparing the lipid extracts from the cells and using them for furth...

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    We usually use HEPES lysis buffer to lysate cells. I want to ask can I use your Free Glycerol Assay Kit (ab65337) and Triglyceride Quantification Kit (ab65336) to measure glycerol level and triglyceride level in the cell extracts after I use this kind of ...

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    Yes, with the Free Glycerol Assay Kit, you can use HEPES for lysis, although the assay buffer provided with the kit is recommended.

    With the Triglyceride Quantification Kit, we would recommend using HEPES and NP-40 since the NP-40 is important f...

    Read More

    Is this kit suitable for samples containing 0.5M TCA?

    As long as you dilute the samples with the assay buffer provided in the kit, there should be no problem with TCA in the samples. Be sure that the pH of your samples is around 5.5 (the pH of the assay buffer). You should adjust the pH of the sample befor...

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    This kit is said usable with urine samples. In fact, whereas there is no problem with the standard curve (in buffer), urine samples spiked with glycerol don't give rise to any color even if spiked with glycerol at a concentration greater than the mid poin...

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    Thank you for your inquiry and your patience with this reply. I will be helping you further as Carolyn is out of the office.
    I spoke to our development scientist about this issue and they thought that the urine contains some compound which may be blo...

    Read More

    Hi,



    I would like to know the reaction that takes place in your glycerol kit, ab65337.

    Which components are a part of it?



    Best

    Thank you for your inquiry.

    The exact description of the components of this kit is proprietary information.

    But I am happy to confirm the way this kit works in more detail:

    The probe that is used in kit ab65337is a highly sen...

    Read More

    1. We would like to know the testing method or test type ?

    Could you send us the instruction for use ?

    2. Can this kit test pure glycerol or impure glycerol in Ester and glycerol?



    We are looking forward to hear...

    Read More

    Thank you for your inquiry.

    Please find the answers to your questions below:

    1.)This Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically...

    Read More

    Hi all,
    I need the msds for the free glycerol assay kit (ab65337) mentioned in the subject otherwise I cannot order this item.
    Thanks.

    Thank you for contacting us.

    I am happy to let you know that the Free Glycerol Assay Kitab65337does not contain any hazardous substances.

    If you wish we could provide the MSDS for DMSO which would state that it is not a hazardous subst...

    Read More

    Thank you for your email.   Our customer would like to receive a replacement with another lot#.   Please check this and arrange a replacement for our customer.  

    Thank you for your reply. I have contacted our Distributors Team and they will issue 1 free of charge replacement of ab65337 on your next Abcam order. I checked the lots we have in stock and this will come from a different lot but the same QC batc...

    Read More

    our customer would like to know if the following kits: #AB83390, AB65342, AB83394, AB65337 can be used for cell culture. I see on your datasheet that only the Pyruvate Assay Kit can be used with biological sample such as cell culture, Do you please ...

    Read More

    All of the kits: ab65337, ab65342, ab83390, ab83394 are suitable for cell and tissue culture supernatants. As well as many other biological fluids like urine, plasma and serum.

    I have bought the product ab65337, a kit for measuring Glycerol in cell medium. I 'm wondering how it's better to store the cell medium that is used for Glycerol measurement. Shall I store the medium at -20°C ? or at + 4°C? Thank you for this information

    Thank you for your enquiry. It would be best to use fresh sample when possible however I know that it may take time to collect all the necessary sample before running an assay. Therefore, I would recommend storing the sample at -20 °C or colder for lo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"