Overview

  • Product nameFree Glycerol Assay KitSee all Glycerol kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 10000 µM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceGlycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.
  • Alternative names
    • Glycerin
    • Glycerine

Applications

Our Abpromise guarantee covers the use of ab65337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Free Glycerol Assay Kit images

  • Glycerol Standard Curve using ab65337.

Protocols

References for Free Glycerol Assay Kit (ab65337)

This product has been referenced in:
  • Carley AN  et al. Multiphasic triacylglycerol dynamics in the intact heart during acute in vivo overexpression of CD36. J Lipid Res 54:97-106 (2013). Functional Studies . Read more (PubMed: 23099442) »

See 1 Publication for this product

Product Wall



Es posible que la cafeína esté interfiriendo a la longitud de onda de lectura de fluorescencia del glicerol (535/587 nm). Como controles alternativos podéis usar isoproterenol (1uM final) o cloroquina.



Ab65337: Este kit puede usarse a partir de extractos celulares. Para ello, comenzar con un número aproximado de ˜2X10^6 células, suspender el pellet en 500ul (o aproximadamente 4 volúmenes) de assay buffer en hielo usando...

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No, I don’t think preparing samples once for both assays is a good idea. For the glycerol assay, you just need cell homogenates, whereas for the triglyceride assay you are actually preparing the lipid extracts from the cells and using them for fu...

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Yes, with the Free Glycerol Assay Kit, you can use HEPES for lysis, although the assay buffer provided with the kit is recommended.

With the Triglyceride Quantification Kit, we would recommend using HEPES and NP-40 since the NP-40 is importan...

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As long as you dilute the samples with the assay buffer provided in the kit, there should be no problem with TCA in the samples. Be sure that the pH of your samples is around 5.5 (the pH of the assay buffer). You should adjust the pH of the sample be...

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Thank you for your inquiry and your patience with this reply. I will be helping you further as Carolyn is out of the office.
I spoke to our development scientist about this issue and they thought that the urine contains some compound which may be ...

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Thank you for your inquiry.

The exact description of the components of this kit is proprietary information.

But I am happy to confirm the way this kit works in more detail:

The probe that is used in kit ab65337is a highly ...

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Thank you for your inquiry.

Please find the answers to your questions below:

1.)This Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatica...

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Thank you for contacting us.

I am happy to let you know that the Free Glycerol Assay Kitab65337does not contain any hazardous substances.

If you wish we could provide the MSDS for DMSO which would state that it is not a hazardous su...

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Thank you for your reply. I have contacted our Distributors Team and they will issue 1 free of charge replacement of ab65337 on your next Abcam order. I checked the lots we have in stock and this will come from a different lot but the same QC b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"