• Product name
    Free Glycerol Assay Kit
    See all Glycerol kits
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 10000 µM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.

  • Tested applications
    Suitable for: Functional Studiesmore details



Our Abpromise guarantee covers the use of ab65337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.

  • Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.

  • a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).



This product has been referenced in:
  • Honek J  et al. Modulation of age-related insulin sensitivity by VEGF-dependent vascular plasticity in adipose tissues. Proc Natl Acad Sci U S A 111:14906-11 (2014). Functional Studies . Read more (PubMed: 25271320) »
  • Lahey R  et al. Dietary fat supply to failing hearts determines dynamic lipid signaling for nuclear receptor activation and oxidation of stored triglyceride. Circulation 130:1790-9 (2014). Read more (PubMed: 25266948) »

See all 4 Publications for this product

Customer reviews and Q&As

Es posible que la cafeína esté interfiriendo a la longitud de onda de lectura de fluorescencia del glicerol (535/587 nm). Como controles alternativos podéis usar isoproterenol (1uM final) o cloroquina.

Ab65337: Este kit puede usarse a partir de extractos celulares. Para ello, comenzar con un número aproximado de ˜2X10^6 células, suspender el pellet en 500ul (o aproximadamente 4 volúmenes) de assay buffer en hielo usando...

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No, I don’t think preparing samples once for both assays is a good idea. For the glycerol assay, you just need cell homogenates, whereas for the triglyceride assay you are actually preparing the lipid extracts from the cells and using them for fu...

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Yes, with the Free Glycerol Assay Kit, you can use HEPES for lysis, although the assay buffer provided with the kit is recommended.

With the Triglyceride Quantification Kit, we would recommend using HEPES and NP-40 since the NP-40 is importan...

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Thank you for your inquiry and your patience with this reply. I will be helping you further as Carolyn is out of the office.
I spoke to our development scientist about this issue and they thought that the urine contains some compound which may be ...

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Thank you for your inquiry.

The exact description of the components of this kit is proprietary information.

But I am happy to confirm the way this kit works in more detail:

The probe that is used in kit ab65337is a highly ...

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Thank you for your inquiry.

Please find the answers to your questions below:

1.)This Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatica...

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Thank you for contacting us.

I am happy to let you know that the Free Glycerol Assay Kitab65337does not contain any hazardous substances.

If you wish we could provide the MSDS for DMSO which would state that it is not a hazardous su...

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Thank you for your reply. I have contacted our Distributors Team and they will issue 1 free of charge replacement of ab65337 on your next Abcam order. I checked the lots we have in stock and this will come from a different lot but the same QC b...

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All of the kits: ab65337, ab65342, ab83390, ab83394 are suitable for cell and tissue culture supernatants. As well as many other biological fluids like urine, plasma and serum.

1-10 of 11 Abreviews or Q&A


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