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Abcam's Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol in various samples.
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Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.
|Glycerol Assay Buffer||WM||1 x 25ml|
|Glycerol Enzyme Mix (lyophilized)||Green||1 vial|
|Glycerol Probe (in DMSO, anhydrous)||Red||1 x 200µl|
|Glycerol Standard (100 mM)||Yellow||1 x 200µl|
Our Abpromise guarantee covers the use of ab65337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"