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Product name
Anti-PFKL antibody
See all PFKL products (5) ...
Description
Rabbit polyclonal to PFKL
Specificity
This antibody is specific for Fructose 6 Phosphate Kinase Liver type (isoenzyme B).
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A KLH conjugated synthetic peptide selected within aa 650~700 of human PFKL (C-Terminal).
Positive control
HepG2 cell lysate and hepatocarcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P: 1/50 - 1/100.
Target
Relevance
PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits.
Cellular localization
Cytoplasmic
Alternative names
- PFK liver isoenzyme antibody
- 6 phosphofructokinase, liver type antibody
- K6PL antibody
see all
Database links
- Entrez Gene: 5211 Human
- Entrez Gene: 18641 Mouse
- Omim: 171860 Human
- SwissProt: P17858 Human
- SwissProt: Q7L2M7 Human
- SwissProt: P12382 Mouse
- Unigene: 255093 Human
Anti-PFKL antibody images:
Western blot - PFKL antibody (ab37583)
Anti-PFKL antibody (ab37583) at 1/100 dilution + HepG2 cell lysate
Predicted band size : 85 kDa
Observed band size : 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PFKL antibody (ab37583)
Staining of Fructose 6 Phosphate Kinase on Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue, using ab37583 at a 1/50 dilution (a peroxidase-conjugated the secondary antibody, followed by AEC staining was used).
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
References for Anti-PFKL antibody (ab37583)
This product has been referenced in:
- Zhong L et al. The Histone Deacetylase Sirt6 Regulates Glucose Homeostasis via Hif1alpha. Cell 140:280-293 (2010). WB; Mouse.Read more (PubMed: 20141841) »
See 1 publication for this product
Publishing research using ab37583? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"