Overview

  • Product nameFumarate Detection Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 nmol/well
  • Assay time
    0h 40m
  • Product overview

    Abcam's Fumarate Detection Kit provides a convenient tool for sensitive detection of Fumarate in a variety of samples. The Fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceFumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy.

Applications

Our Abpromise guarantee covers the use of ab102516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Fumarate Detection Kit images

  • Fumarate measured in biological fluids, background signal subtracted (duplicates +/- SD).

  • Fumarate measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Fumarate measured in cell lysates, background signal subtracted (duplicates +/- SD).

  • Fumarate measured in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).

  • Fumurate Standard Curve

Protocols

References for Fumarate Detection Kit (ab102516)

ab102516 has not yet been referenced specifically in any publications.

Product Wall

As Fumarate is a chemical and not a protein, it's detection is not limited by species specificity as it would be the case if you would like to detect a protein.
In addition, as blood serum is blood plasma without clotting factors, and plasma has be...

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The final colour development should be pink but the reaction mix should not be pink to begin with. Only once you add this to your samples, will the reaction initiate and lead to the pink colour development.

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products.

Ho...

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Thank you very much for your interest in our kit ab102516 for fumarate detection.

The laboratory confirmed that we haven’t tried this kit with microbial samples in-house and neither do we have any researcher feedback from such use. So a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"