Furin antibody (ab3467)

Product Name 

Furin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Furin

Immunogen 

Synthetic peptide: RGERTAFIKDQSAL, corresponding to amino acids 780-793 of Human Furin(Peptide available as ab4989.)
BLAST 'RGERTAFIKDQSAL' with or

Reacts with 

Hu, Ms, Dog

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Specificity 

Detects Furin from canine and mouse cells as well as transfected human Furin.
This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Immunoprecipitation: 1/100.

Western Blot: 1/1000.
Immunofluorescence: assay dependent.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects an ~100 kDa protein representing Furin in BSC-40 cells transfected with the human Furin gene.

This antibody detects human Furin transfected into 3T3 cells.

Immunofluorescence staining of endogenous Furin convertase in 3T3 cells yields a pattern consistent with paranuclear staining.

Cellular localization 

Golgi apparatus; trans-Golgi network.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment.

Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment.

Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS, 1 mg/ml BSA and 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Furin antibody images, references, products related to ab3467 and other tools.

Furin antibody images:

References for Furin antibody (ab3467)

  This product has been used in: (two most recent references)

Sumara G et al. Regulation of PKD by the MAPK p38delta in insulin secretion and glucose homeostasis. Cell 136:235-48 (2009). ICC/IF; Mouse. PubMed: 19135240

Huynh TT et al. Effect of ultraviolet radiation on the expression of pp38MAPK and furin in human keratinocyte-derived cell lines. Photodermatol Photoimmunol Photomed 25:20-9 (2009). WB; Human. PubMed: 19152512

See all 5 publication references for this product.


If you publish research using ab3467 please let us know so that we can cite the reference on this datasheet.

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