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ab4989 |
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ab6032 |
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Read our guarantee »Publishing research using ab3467? Please let us know so that we can cite the reference in this datasheet
ab3467 has been referenced in 5 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.
All lanes : Anti-Furin antibody (ab3467) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 87 kDa
Observed band size : 63 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 seconds
The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.
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