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Product Name
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Fyn antibody [FYN-01]
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See all Fyn antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [FYN-01] to Fyn
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Immunogen
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Recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases.
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Tested applications
(see key)
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ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.
The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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FYN-01
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Isotype
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IgG
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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Purified from hybridoma culture supernatant by protein A affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Fyn antibody [FYN-01] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Fyn antibody [FYN-01] (ab1881)
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This product has been used in: (two most recent references)
Mitchell LA et al. Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa. Mol Hum Reprod 14:235-43 (2008). PubMed: 18245108
Hauri-Hohl MM et al. Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation. Blood 109:4080-8 (2007). PubMed: 17213290
If you publish research using ab1881 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Fyn
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