Fyn antibody [Y303] (ab32022)

Product Name 

Fyn antibody [Y303]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y303] to Fyn

Immunogen 

Synthetic peptide corresponding to residues near the N terminal of human Fyn

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Skeletal muscle tissue, bone marrow, spinal cord samples, or heart have LOW levels of Fyn expression.

Epitope 

ab32022 reacts with an epitope located in the N terminal region of Fyn.

Tested applications 
(see key)

ICC, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/50 - 1/100.
IHC-P: 1/50 - 1/100.

WB: 1/2000. Detects a band of approximately 59 kDa (predicted molecular weight: 60 kDa).

Is unsuitable for FACS or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

NIH 3T3 cell lysate and human breast carcinoma

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.

The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y303

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for Fyn antibody [Y303] images, references, products related to ab32022 and other tools.

Fyn antibody [Y303] images:

References for Fyn antibody [Y303] (ab32022)

  This product has been used in:

Mill P et al. Palmitoylation regulates epidermal homeostasis and hair follicle differentiation. PLoS Genet 5:e1000748 (2009). IHC-P; Mouse. PubMed: 19956733

If you publish research using ab32022 please let us know so that we can cite the reference on this datasheet.

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