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Product name
Anti-Fyn antibody
See all Fyn products (13) ...
Description
Goat polyclonal to Fyn
Specificity
This antibody is expected to recognise all three reported isoforms (as represented by NP_002028; NP_694592; NP_694593).
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: VQCKDKEATKLTE, corresponding to N terminal amino acids 3-15 of Fyn.
VQCKDKEATKLTE
Epitope
From N Terminus.
Positive control
Jurkat, A431 and NIH3T3 lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Western blot - Anti-Fyn antibody (ab802)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 64 kDa).
Target
Function
Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
Tissue specificity
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Cellular localization
Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
Target information above from: UniProt accessionP06241
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
see all
Database links
- Entrez Gene: 2534 Human
- Entrez Gene: 14360 Mouse
- Entrez Gene: 25150 Rat
- Omim: 137025 Human
- SwissProt: P06241 Human
- SwissProt: P39688 Mouse
- SwissProt: Q62844 Rat
- Unigene: 390567 Human
see all
Anti-Fyn antibody images:
References for Anti-Fyn antibody (ab802)
ab802 has not yet been referenced specifically in any publications.
Publishing research using ab802? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"