Fyn (phospho Y530) antibody (ab53690)

Product Name 

Fyn (phospho Y530) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Fyn (phospho Y530)

Immunogen 

Synthetic phosphopeptide derived from human Fyn around the phosphorylation site of tyrosine 530 (P-Q-Y^P-Q-P).

Reacts with 
(species key)

Hu, Ms

Specificity 

ab53690 detects endogenous levels of Fyn only when phosphorylated at tyrosine 530.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293 cell extracts treated with H²O² (100uM, 15mins).

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.

The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab53690 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

See below for Fyn (phospho Y530) antibody images, references, products related to ab53690 and other tools.

Fyn (phospho Y530) antibody images:

Customer reviews (feedback) regarding Fyn (phospho Y530) antibody

Customer FAQs regarding Fyn (phospho Y530) antibody

Protocols for Fyn (phospho Y530) antibody

Price and availability of products related to Fyn (phospho Y530) antibody

Fyn (phospho Y530) antibody - related products: