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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Fyn (phospho Y530) antibody
See all Fyn products (13) ...
Description
Rabbit polyclonal to Fyn (phospho Y530)
Specificity
ab53690 detects endogenous levels of Fyn only when phosphorylated at tyrosine 530.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic phosphopeptide derived from human Fyn around the phosphorylation site of tyrosine 530 (P-Q-YP-Q-P).
Positive control
293 cell extracts treated with H2O2 (100uM, 15mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53690 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Western blot - Fyn (phospho Y530) antibody (ab53690)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
Tissue specificity
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Cellular localization
Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
Target information above from: UniProt accessionP06241
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
see all
Database links
- Entrez Gene: 2534 Human
- Entrez Gene: 14360 Mouse
- Omim: 137025 Human
- SwissProt: P06241 Human
- SwissProt: P39688 Mouse
- Unigene: 390567 Human
- Unigene: 4848 Mouse
Anti-Fyn (phospho Y530) antibody images:
Western blot - Fyn (phospho Y530) antibody (ab53690)
All lanes : Anti-Fyn (phospho Y530) antibody (ab53690) at 1/500 dilution
Lane 1 : 293 cell extract treated
with H2O2 (100uM, 15mins)
Lane 2 : 293 cell extract treated
with H2O2 (100uM, 15mins) with immunising peptide
Predicted band size : 60 kDa
Observed band size : 60 kDa
References for Anti-Fyn (phospho Y530) antibody (ab53690)
This product has been referenced in:
- Kim S & Kunapuli SP Negative Regulation of Gq-mediated Pathways in Platelets by G12/13 Pathways through Fyn Kinase. J Biol Chem 286:24170-9 (2011). WB; Human.Read more (PubMed: 21592972) »
See 1 publication for this product
Publishing research using ab53690? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"