G-CSF protein (Active) (ab73371)
Constituents: 10mM Hydrochloric Acid, Mannitol, Tween 20, L-arginine, phenylalanine and methionine. pH 6.5
Our Abpromise guarantee covers the use of ab73371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution. The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|WB||WB: Use at an assay dependent concentration. Ab73371 can be used as a WB positive control in conjunction with ab9691.|
- Colony stimulating factor 3 (granulocyte)CSF 3CSF beta
- CSF3CSF3_HUMANCsfgFilgrastimG-CSFGCSAGCSFGranulocyte colony stimulating factorGranulocyte colony-stimulating factorLenograstimMacrophage granulocyte inducer 2MGC45931MGI 2Pluripoietin
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
G-CSF protein (Active) images
Anti-G-CSF antibody (ab9691) at 1 µg/ml +
G-CSF protein (Active) (ab73371) at 0.01 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
References for G-CSF protein (Active) (ab73371)
ab73371 has not yet been referenced specifically in any publications.