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Overview

  • Product nameG-CSF protein (Active)See all G-CSF proteins and peptides ...
  • Protein descriptionRecombinant full length human G-CSF; a single, glycosylated, polypeptide chain containing 174 amino acids, 20kDa. SwissProt P09919.
  • Expression hostCHO

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab73371 was purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • Biological activitySpecific Activity of 1.27 x 108 IU/mg.
  • FormLyophilised:Reconstitute in sterile 18MOhm/cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10mM Hydrochloric Acid, Mannitol, Tween 20, L-arginine, phenylalanine and methionine. pH 6.5
  • Concentration information loading...
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Sequence notesThe sequence of the first five N-terminal amino acids was determined and was found to be Thr-Pro-Leu-Gly-Pro.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73371 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent concentration. Ab73371 can be used as a WB positive control in conjunction with ab9691.

    Protein info

    • Alternative names
        Colony stimulating factor 3 (granulocyte)CSF 3CSF beta
        CSF3CSF3_HUMANCsfgFilgrastimG-CSFGCSAGCSFGranulocyte colony stimulating factorGranulocyte colony-stimulating factorLenograstimMacrophage granulocyte inducer 2MGC45931MGI 2Pluripoietin
      see all
  • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P09919 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    G-CSF protein (Active) images

    • Western blot
      Western blot
      Anti-G-CSF antibody (ab9691) at 1 µg/ml + G-CSF protein (Active) (ab73371) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 2 minutes

    References for G-CSF protein (Active) (ab73371)

    ab73371 has not yet been referenced specifically in any publications.

    Publishing research using ab73371 ? Please let us know so that we can cite the reference in this datasheet

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