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Overview

  • Product nameG-CSF protein (Active)See all G-CSF proteins and peptides ...
  • Protein descriptionRecombinant full length human G-CSF protein (amino acids 30-207) expressed in modified human 293 cells (Swiss-Prot P09919).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityThe ED50 of ab83995 is typically 0.01 - 0.03 ng/ml as measured in a cell proliferation assay using a murine myeloblastic M-NFS-60 cell line.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: ATPLGPASSLPQSFLLKCLEQVRKIQGDG AALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSS CPS QALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTL QLDVADFATTIWQQMEELG MAPALQPTQGAMPAFASAF QRRAGGVLVASHLQSFLEVSYRVLRHLAQP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83995 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab83995 migrates as a band between 15 and 20 kDa in SDS-PAGE. This compares with the predicted molecular mass of 18.7 kDa.

    pI: ab83995 separates into a number of isoforms with a pI between 5.4 and 6.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified G-CSF that has a predicted pI of 5.65.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Colony stimulating factor 3 (granulocyte)CSF 3CSF beta
        CSF3CSF3_HUMANCsfgFilgrastimG-CSFGCSAGCSFGranulocyte colony stimulating factorGranulocyte colony-stimulating factorLenograstimMacrophage granulocyte inducer 2MGC45931MGI 2Pluripoietin
      see all
  • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P09919 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for G-CSF protein (Active) (ab83995)

    ab83995 has not yet been referenced specifically in any publications.

    Publishing research using ab83995 ? Please let us know so that we can cite the reference in this datasheet

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