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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - GAB1 antibody (ab27439)
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Product Name
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GAB1 antibody
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See all GAB1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GAB1
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Immunogen
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A synthetic peptide from C terminal of human Gab1.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Mk
(Due to sequence homology)
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Specificity
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ab27439 recognises GAB1.
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Tested applications
(see key)
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IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/50 for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min and then cool at room temperature for 20 min.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 77 kDa but band is known to be observed at 115kDa due to post translational modifications.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293 cells, astrocytoma.
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Cellular localization
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Cytoplasmic (probable).
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Research areas
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Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAB1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GAB1 antibody images, references, products related to ab27439 and other tools.
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GAB1 antibody images:
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 Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - GAB1 antibody (ab27439)
Paraffin embedded formalin fixed human astrocytoma stained with anti Gab1 antibody (ab27439).
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Search PubMed (MEDLINE) for references to GAB1
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GAB1 antibody - more information
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Customer reviews (feedback) regarding GAB1 antibody |
Customer FAQs regarding GAB1 antibody |
Protocols for GAB1 antibody |
Price and availability of products related to GAB1 antibody |
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GAB1 antibody - related products:
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GAB1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GAB1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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