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All lanes : Anti-GAB2 (phospho S159) antibody (ab78208) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells, treated
with serum (20%, 15mins)
Lane 2 : Extracts from HT-29 cells, treated
with serum (20%, 15mins) plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 74 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
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