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Western blot - GAB2 (phospho S159) antibody (ab78208)
(enlarge)
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Product Name
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GAB2 (phospho S159) antibody
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See all GAB2 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GAB2 (phospho S159)
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Immunogen
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Synthesized phosphopeptide derived from human GAB2 around the phosphorylation site of serine 159 (K-S-SP-A-P).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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This antibody detects endogenous levels of GAB2 only when phosphorylated at serine 159.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HT-29 cells treated with serum (20%, 15mins).
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Cellular localization
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Cytoplasm. Cell membrane
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Research areas
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Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.
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GAB2 (phospho S159) antibody (ab78208) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAB2 (phospho S159) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GAB2 (phospho S159) antibody images, references, products related to ab78208 and other tools.
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GAB2 (phospho S159) antibody images:
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 Western blot - GAB2 (phospho S159) antibody (ab78208)
All lanes : GAB2 (phospho S159) antibody (ab78208) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells, treated
with serum (20%, 15mins)
Lane 2 : Extracts from HT-29 cells, treated
with serum (20%, 15mins) plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 74 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to GAB2
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GAB2 (phospho S159) antibody - more information
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GAB2 (phospho S159) antibody - related products:
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GAB2 (phospho S159) antibody - other tools:
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