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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-GAB2 (phospho Y643) antibody
See all GAB2 products (9) ...
Description
Rabbit polyclonal to GAB2 (phospho Y643)
Specificity
This antibody detects endogenous levels of GAB2 only when phosphorylated at tyrosine 643.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized phosphopeptide derived from human GAB2 around the phosphorylation site of tyrosine 643 (V-D-YP-V-Q).
Positive control
Extracts from Jurkat cells. Human brain tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Relevance
Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.
Cellular localization
Cytoplasm. Cell membrane
Alternative names
- GRB2 associated binder 2 pp100 antibody
- GAB 2 antibody
- Grb 2 associated binder 2 antibody
see all
Database links
- Entrez Gene: 9846 Human
- Entrez Gene: 14389 Mouse
- Entrez Gene: 84477 Rat
- Omim: 606203 Human
- SwissProt: Q9UQC2 Human
- SwissProt: Q9Z1S8 Mouse
- SwissProt: Q9EQH1 Rat
Anti-GAB2 (phospho Y643) antibody images:
Western blot - GAB2 (phospho Y643) antibody (ab78262)
All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins)
Lane 2 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 74 kDa
Observed band size : 74 kDa
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAB2 (phospho Y643) antibody (ab78262)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
References for Anti-GAB2 (phospho Y643) antibody (ab78262)
ab78262 has not yet been referenced specifically in any publications.
Publishing research using ab78262? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"